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Folate and vitamin B12 status; associations with maternal glucose and neonatal DNA methylation sites related to dysglycaemia, in pregnant women with obesity.

Folate and vitamin B12 status; associations with maternal glucose and neonatal DNA methylation sites related to dysglycaemia, in pregnant women with obesity.
Folate and vitamin B12 status; associations with maternal glucose and neonatal DNA methylation sites related to dysglycaemia, in pregnant women with obesity.

Recent studies implicate maternal gestational diabetes mellitus (GDM) in differential methylation of infant DNA. Folate and vitamin B12 play a role in DNA methylation, and these vitamins may also influence GDM risk. The aims of this study were to determine folate and vitamin B12 status in obese pregnant women and investigate associations between folate and vitamin B12 status, maternal dysglycaemia and neonatal DNA methylation at cytosine-phosphate-guanine sites previously observed to be associated with dysglycaemia. Obese pregnant women who participated in the UK Pregnancies Better Eating and Activity Trial were included. Serum folate and vitamin B12 were measured at the oral glucose tolerance test (OGTT) visit. Cord blood DNA methylation was assessed using the Infinium MethylationEPIC BeadChip. Regression models with adjustment for confounders were used to examine associations. Of the 951 women included, 356 (37.4%) were vitamin B12 deficient, and 44 (4.6%) were folate deficient. Two-hundred and seventy-one women (28%) developed GDM. Folate and vitamin B12 concentrations were not associated with neonatal DNA methylation. Higher folate was positively associated with 1-h plasma glucose after OGTT (β = 0.031, 95% CI 0.001-0.061, p = 0.045). There was no relationship between vitamin B12 and glucose concentrations post OGTT or between folate or vitamin B12 and GDM. In summary, we found no evidence to link folate and vitamin B12 status with the differential methylation of neonatal DNA previously observed in association with dysglycaemia. We add to the evidence that folate status may be related to maternal glucose homoeostasis although replication in other maternal cohorts is required for validation.

DNA methylation, Folate, gestational diabetes mellitus, maternal obesity, vitamin B12
Weelden, Wenneke van
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Seed, Paul T.
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Antoun, Elie
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Godfrey, Keith
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Kitaba, Negusse
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Lillycrop, Karen
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Dalrymple, Kathryn V.
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Sobczyńska-Malefora, Agata
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Painter, Rebecca C.
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Poston, Lucilla
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White, Sara L.
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Flynn, Angela
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Weelden, Wenneke van
fbd90fd3-b2c5-4ac6-9fb0-c84eae8ee870
Seed, Paul T.
d3eda3f4-0e4c-4c17-9efd-dc75b583e79a
Antoun, Elie
10fc5678-b33c-4410-977d-b11234031791
Godfrey, Keith
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Kitaba, Negusse
5e35ae4a-edaa-4b78-bcb6-00628c3b6e83
Lillycrop, Karen
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Dalrymple, Kathryn V.
8ef94198-4e90-44a9-b77d-19d35d013cde
Sobczyńska-Malefora, Agata
8887e6df-c8e4-4b94-8788-a9645c421627
Painter, Rebecca C.
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Poston, Lucilla
916aced2-462e-445f-9efa-83ed4b7b3a9f
White, Sara L.
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Flynn, Angela
02bcba40-29da-4e1d-a94e-cc3fac438183

Weelden, Wenneke van, Seed, Paul T., Antoun, Elie, Godfrey, Keith, Kitaba, Negusse, Lillycrop, Karen, Dalrymple, Kathryn V., Sobczyńska-Malefora, Agata, Painter, Rebecca C., Poston, Lucilla, White, Sara L. and Flynn, Angela (2021) Folate and vitamin B12 status; associations with maternal glucose and neonatal DNA methylation sites related to dysglycaemia, in pregnant women with obesity. Journal of Developmental Origins of Health and Disease. (doi:10.1017/S2040174421000246).

Record type: Article

Abstract

Recent studies implicate maternal gestational diabetes mellitus (GDM) in differential methylation of infant DNA. Folate and vitamin B12 play a role in DNA methylation, and these vitamins may also influence GDM risk. The aims of this study were to determine folate and vitamin B12 status in obese pregnant women and investigate associations between folate and vitamin B12 status, maternal dysglycaemia and neonatal DNA methylation at cytosine-phosphate-guanine sites previously observed to be associated with dysglycaemia. Obese pregnant women who participated in the UK Pregnancies Better Eating and Activity Trial were included. Serum folate and vitamin B12 were measured at the oral glucose tolerance test (OGTT) visit. Cord blood DNA methylation was assessed using the Infinium MethylationEPIC BeadChip. Regression models with adjustment for confounders were used to examine associations. Of the 951 women included, 356 (37.4%) were vitamin B12 deficient, and 44 (4.6%) were folate deficient. Two-hundred and seventy-one women (28%) developed GDM. Folate and vitamin B12 concentrations were not associated with neonatal DNA methylation. Higher folate was positively associated with 1-h plasma glucose after OGTT (β = 0.031, 95% CI 0.001-0.061, p = 0.045). There was no relationship between vitamin B12 and glucose concentrations post OGTT or between folate or vitamin B12 and GDM. In summary, we found no evidence to link folate and vitamin B12 status with the differential methylation of neonatal DNA previously observed in association with dysglycaemia. We add to the evidence that folate status may be related to maternal glucose homoeostasis although replication in other maternal cohorts is required for validation.

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Accepted/In Press date: 9 April 2021
e-pub ahead of print date: 11 May 2021
Keywords: DNA methylation, Folate, gestational diabetes mellitus, maternal obesity, vitamin B12

Identifiers

Local EPrints ID: 448411
URI: http://eprints.soton.ac.uk/id/eprint/448411
PURE UUID: 45d844dd-8cc9-442a-92aa-f9b6c2f61319
ORCID for Keith Godfrey: ORCID iD orcid.org/0000-0002-4643-0618
ORCID for Negusse Kitaba: ORCID iD orcid.org/0000-0001-7518-9096
ORCID for Karen Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489

Catalogue record

Date deposited: 21 Apr 2021 16:35
Last modified: 26 Nov 2021 05:58

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Contributors

Author: Wenneke van Weelden
Author: Paul T. Seed
Author: Elie Antoun
Author: Keith Godfrey ORCID iD
Author: Negusse Kitaba ORCID iD
Author: Karen Lillycrop ORCID iD
Author: Kathryn V. Dalrymple
Author: Agata Sobczyńska-Malefora
Author: Rebecca C. Painter
Author: Lucilla Poston
Author: Sara L. White
Author: Angela Flynn

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