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Acoustofluidic device for acoustic capture of Bacillus anthracis spore analogues at low concentration

Acoustofluidic device for acoustic capture of Bacillus anthracis spore analogues at low concentration
Acoustofluidic device for acoustic capture of Bacillus anthracis spore analogues at low concentration

A portable device for the rapid concentration of Bacillus subtilis var niger spores, also known as Bacillus globigii (BG), using a thin-reflector acoustofluidic configuration is described. BG spores form an important laboratory analog for the Bacillus anthracis spores, a serious health and bioterrorism risk. Existing systems for spore detection have limitations on detection time and detection that will benefit from the combination with this technology. Thin-reflector acoustofluidic devices can be cheaply and robustly manufactured and provide a more reliable acoustic force than previously explored quarter-wave resonator systems. The system uses the acoustic forces to drive spores carried in sample flows of 30 ml/h toward an antibody functionalized surface, which captures and immobilizes them. In this implementation, spores were fluorescently labeled and imaged. Detection at concentrations of 100 CFU/ml were demonstrated in an assay time of 10 min with 60% capture. We envisage future systems to incorporate more advanced detection of the concentrated spores, leading to rapid, sensitive detection in the presence of significant noise.

Acoustic capture, anthrax spore analogues
0001-4966
4228-4238
Plazonic, Filip
3b12ec91-76d8-465c-90e4-ae2af8127be1
Fisher, Adam
0cbfd7a8-a475-45eb-bf51-3f5377645c51
Carugo, Dario
cf740d40-75f2-4073-9c6e-6fcf649512ca
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Plazonic, Filip
3b12ec91-76d8-465c-90e4-ae2af8127be1
Fisher, Adam
0cbfd7a8-a475-45eb-bf51-3f5377645c51
Carugo, Dario
cf740d40-75f2-4073-9c6e-6fcf649512ca
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d

Plazonic, Filip, Fisher, Adam, Carugo, Dario, Hill, Martyn and Glynne-Jones, Peter (2021) Acoustofluidic device for acoustic capture of Bacillus anthracis spore analogues at low concentration. The Journal of The Acoustical Society of America, 149 (6), 4228-4238. (doi:10.1121/10.0005278).

Record type: Article

Abstract

A portable device for the rapid concentration of Bacillus subtilis var niger spores, also known as Bacillus globigii (BG), using a thin-reflector acoustofluidic configuration is described. BG spores form an important laboratory analog for the Bacillus anthracis spores, a serious health and bioterrorism risk. Existing systems for spore detection have limitations on detection time and detection that will benefit from the combination with this technology. Thin-reflector acoustofluidic devices can be cheaply and robustly manufactured and provide a more reliable acoustic force than previously explored quarter-wave resonator systems. The system uses the acoustic forces to drive spores carried in sample flows of 30 ml/h toward an antibody functionalized surface, which captures and immobilizes them. In this implementation, spores were fluorescently labeled and imaged. Detection at concentrations of 100 CFU/ml were demonstrated in an assay time of 10 min with 60% capture. We envisage future systems to incorporate more advanced detection of the concentrated spores, leading to rapid, sensitive detection in the presence of significant noise.

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V2 Spore paper - Accepted Manuscript
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Accepted/In Press date: 27 May 2021
e-pub ahead of print date: 15 June 2021
Published date: 16 June 2021
Additional Information: Funding Information: Support under the Engineering and Physical Sciences Research Council (UK; EPSRC) Fellowship No. EP/ L025035/1 is gratefully acknowledged. The authors would like to thank Martin McDonnell and DSTL at Porton Down for providing the BG spores and expertise in slide functionalization. All relevant data have been included in this paper. Publisher Copyright: © 2021 Acoustical Society of America.
Keywords: Acoustic capture, anthrax spore analogues

Identifiers

Local EPrints ID: 449639
URI: http://eprints.soton.ac.uk/id/eprint/449639
ISSN: 0001-4966
PURE UUID: a6f9ac53-a571-4425-ae7a-3b99371450f3
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953

Catalogue record

Date deposited: 10 Jun 2021 16:30
Last modified: 30 Jun 2022 04:03

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Contributors

Author: Filip Plazonic
Author: Adam Fisher
Author: Dario Carugo
Author: Martyn Hill ORCID iD

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