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Native-like SARS-CoV-2 spike Glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine

Native-like SARS-CoV-2 spike Glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine
Native-like SARS-CoV-2 spike Glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine

Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation, and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirm the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.

2374-7943
594-602
Watanabe, Yasunori
8c0ee4af-a293-4de5-9036-3ce2051b380c
Mendonça, Luiza
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Allen, Elizabeth R
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Howe, Andrew
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Lee, Mercede
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Allen, Joel D
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Chawla, Himanshi
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Pulido, David
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Donnellan, Francesca
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Davies, Hannah
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Ulaszewska, Marta
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Belij-Rammerstorfer, Sandra
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Morris, Susan
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Krebs, Anna-Sophia
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Dejnirattisai, Wanwisa
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Mongkolsapaya, Juthathip
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Supasa, Piyada
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Screaton, Gavin R
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Green, Catherine M
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Lambe, Teresa
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Zhang, Peijun
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Gilbert, Sarah C
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Crispin, Max
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Watanabe, Yasunori
8c0ee4af-a293-4de5-9036-3ce2051b380c
Mendonça, Luiza
c78a7dd5-9019-43e8-bb71-6ffa80ef003a
Allen, Elizabeth R
9776e2b7-1648-458c-879a-14a0b9b8ce7c
Howe, Andrew
e648d779-cf91-4cb7-82c5-b8180f938eda
Lee, Mercede
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Allen, Joel D
c89d5569-7659-4835-b535-c9586e956b3a
Chawla, Himanshi
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Pulido, David
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Donnellan, Francesca
cbc7bc14-2382-4184-9d21-8935d6e02376
Davies, Hannah
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Ulaszewska, Marta
c45eda05-8312-40b9-a0e4-4362af7cc43b
Belij-Rammerstorfer, Sandra
a1f3ac56-807a-4221-9a1f-053fb02470b3
Morris, Susan
25befade-574f-465a-b483-92b40efc56d8
Krebs, Anna-Sophia
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Dejnirattisai, Wanwisa
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Mongkolsapaya, Juthathip
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Supasa, Piyada
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Screaton, Gavin R
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Green, Catherine M
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Lambe, Teresa
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Zhang, Peijun
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Gilbert, Sarah C
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Crispin, Max
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Watanabe, Yasunori, Mendonça, Luiza, Allen, Elizabeth R, Howe, Andrew, Lee, Mercede, Allen, Joel D, Chawla, Himanshi, Pulido, David, Donnellan, Francesca, Davies, Hannah, Ulaszewska, Marta, Belij-Rammerstorfer, Sandra, Morris, Susan, Krebs, Anna-Sophia, Dejnirattisai, Wanwisa, Mongkolsapaya, Juthathip, Supasa, Piyada, Screaton, Gavin R, Green, Catherine M, Lambe, Teresa, Zhang, Peijun, Gilbert, Sarah C and Crispin, Max (2021) Native-like SARS-CoV-2 spike Glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine. ACS Central Science, 7 (4), 594-602. (doi:10.1021/acscentsci.1c00080).

Record type: Article

Abstract

Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation, and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirm the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.

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Published date: 28 April 2021
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Identifiers

Local EPrints ID: 449784
URI: http://eprints.soton.ac.uk/id/eprint/449784
DOI: doi:10.1021/acscentsci.1c00080
ISSN: 2374-7943
PURE UUID: a7cb5325-6062-4761-bc73-21cbcd105857
ORCID for Joel D Allen: ORCID iD orcid.org/0000-0003-2547-968X
ORCID for Himanshi Chawla: ORCID iD orcid.org/0000-0001-9828-6593
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 17 Jun 2021 16:31
Last modified: 02 May 2024 02:00

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Contributors

Author: Yasunori Watanabe
Author: Luiza Mendonça
Author: Elizabeth R Allen
Author: Andrew Howe
Author: Mercede Lee
Author: Joel D Allen ORCID iD
Author: Himanshi Chawla ORCID iD
Author: David Pulido
Author: Francesca Donnellan
Author: Hannah Davies
Author: Marta Ulaszewska
Author: Sandra Belij-Rammerstorfer
Author: Susan Morris
Author: Anna-Sophia Krebs
Author: Wanwisa Dejnirattisai
Author: Juthathip Mongkolsapaya
Author: Piyada Supasa
Author: Gavin R Screaton
Author: Catherine M Green
Author: Teresa Lambe
Author: Peijun Zhang
Author: Sarah C Gilbert
Author: Max Crispin ORCID iD

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