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Development of genetic tools for the redbait species Pyura herdmani and P. stolonifera, important bioengineers along African coastlines

Development of genetic tools for the redbait species Pyura herdmani and P. stolonifera, important bioengineers along African coastlines
Development of genetic tools for the redbait species Pyura herdmani and P. stolonifera, important bioengineers along African coastlines
The development of new genetic resources is key for biological conservation in an era when the preservation of biodiversity is crucial. Here, we report the development of microsatellites and mitochondrial markers for the redbait species Pyura herdmani and P. stolonifera, both important bioengineers along African coastlines. Specifically, we identified microsatellites by means of pyrosequencing and variable regions in the mitochondrial genome using RAD-seq libraries that were generated with Illumina sequencing. A total of 4 339 putative microsatellites were found, of which 32 were tested on individuals collected along the southeast coast of South Africa. Of those, five microsatellites cross-amplified consistently in both species, and these were tested on a total of 260 samples. Additionally, we mapped the partial mitochondrial genome of a small number of individuals from both species and identified the most-variable regions by comparing a small number of individuals from all regions where these species have been recorded (including western South Africa and northwest Africa). The genetic markers developed here can be used to conduct population genetic studies, to monitor range shifts under contemporary climate change, and to help in preserving ecosystem functioning along African coastlines.
Illumina sequencing, RAD-seq, ascidians, microsatellites, mitochondrial markers, mitogenome, short tandem repeats
1814-232X
1-7
Dinoi, A
52c756f7-e197-4733-98f4-b90a5159a5db
Rius, M
c4e88345-4b4e-4428-b4b2-37229155f68d
Tine, M
71c71e72-d407-488f-b7ab-88859c77a305
Teske, Pr
a85ccc74-7aa3-424c-bf48-2caaecbe0e70
Dinoi, A
52c756f7-e197-4733-98f4-b90a5159a5db
Rius, M
c4e88345-4b4e-4428-b4b2-37229155f68d
Tine, M
71c71e72-d407-488f-b7ab-88859c77a305
Teske, Pr
a85ccc74-7aa3-424c-bf48-2caaecbe0e70

Dinoi, A, Rius, M, Tine, M and Teske, Pr (2021) Development of genetic tools for the redbait species Pyura herdmani and P. stolonifera, important bioengineers along African coastlines. African Journal of Marine Science, 43 (2), 1-7. (doi:10.2989/1814232X.2021.1925346).

Record type: Article

Abstract

The development of new genetic resources is key for biological conservation in an era when the preservation of biodiversity is crucial. Here, we report the development of microsatellites and mitochondrial markers for the redbait species Pyura herdmani and P. stolonifera, both important bioengineers along African coastlines. Specifically, we identified microsatellites by means of pyrosequencing and variable regions in the mitochondrial genome using RAD-seq libraries that were generated with Illumina sequencing. A total of 4 339 putative microsatellites were found, of which 32 were tested on individuals collected along the southeast coast of South Africa. Of those, five microsatellites cross-amplified consistently in both species, and these were tested on a total of 260 samples. Additionally, we mapped the partial mitochondrial genome of a small number of individuals from both species and identified the most-variable regions by comparing a small number of individuals from all regions where these species have been recorded (including western South Africa and northwest Africa). The genetic markers developed here can be used to conduct population genetic studies, to monitor range shifts under contemporary climate change, and to help in preserving ecosystem functioning along African coastlines.

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Accepted/In Press date: 1 December 2020
e-pub ahead of print date: 15 June 2021
Keywords: Illumina sequencing, RAD-seq, ascidians, microsatellites, mitochondrial markers, mitogenome, short tandem repeats

Identifiers

Local EPrints ID: 450297
URI: http://eprints.soton.ac.uk/id/eprint/450297
ISSN: 1814-232X
PURE UUID: 3fa4da1d-2da6-43d4-82da-e23a438d01f3

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Date deposited: 21 Jul 2021 16:30
Last modified: 25 Nov 2021 23:09

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Contributors

Author: A Dinoi
Author: M Rius
Author: M Tine
Author: Pr Teske

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