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Placental 13C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight

Placental 13C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight
Placental 13C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight
Background: fetal docosahexaenoic acid (DHA) supply relies on preferential transplacental transfer, which is regulated by placental DHA lipid metabolism. Maternal hyperglycemia and obesity associate with higher birthweight and fetal DHA insufficiency but the role of placental DHA metabolism is unclear.

Methods: explants from 17 term placenta were incubated with 13C-labeled DHA for 48h, at 5 or 10 mmol/L glucose treatment, and the production of seventeen individual newly synthesized 13C-DHA labeled lipids quantified by liquid chromatography mass spectrometry.

Results: maternal BMI positively associated with 13C-DHA-labeled diacylglycerols, triacylglycerols, lysophospholipids, phosphatidylcholine and phosphatidylethanolamine plasmalogens, while maternal fasting glycemia positively associated with five 13C-DHA triacylglycerols. In turn, 13C-DHA-labeled phospholipids and triacylglycerols positively associated with birthweight centile. In-vitro glucose treatment increased most 13C-DHA-lipids, but decreased 13C-DHA phosphatidylethanolamine plasmalogens. However, with increasing maternal BMI, the magnitude of the glucose treatment induced increase in 13C-DHA phosphatidylcholine and 13C-DHA lysophospholipids was curtailed, with further decline in 13C-DHA phosphatidylethanolamine plasmalogens. Conversely, with increasing birthweight centile glucose treatment induced increases in 13C-DHA triacylglycerols were exaggerated, while glucose treatment induced decreases in 13C-DHA phosphatidylethanolamine plasmalogens were diminished.

Conclusions: maternal BMI and glycemia increased the production of different placental DHA lipids implying impact on different metabolic pathways. Glucose-induced elevation in placental DHA metabolism is moderated with higher maternal BMI. In turn, findings of associations between many DHA lipids with birthweight suggest that BMI and glycemia promote fetal growth partly through changes in placental DHA metabolism.
1076-1551
Watkins, Oliver C.
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Selvam, Preben
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Pillai, Reshma Appukuttan
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Cracknell, Victoria, Kathryn Bonnell
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Yong, Hannah E.J.
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Sharma, Neha
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Cazenave-Gassiot, Amaury
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Bendt, Anne K.
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Godfrey, Keith
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Lewis, Rohan
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Wenk, Markus R.
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Chan, Shiao-Yng
3c9d8970-2cc4-430a-86a7-96f6029a5293
Watkins, Oliver C.
985df9b8-72d6-4f32-9ee3-54553c990fdc
Selvam, Preben
989ffd8a-e214-43b5-a3ce-a814fc491ee7
Pillai, Reshma Appukuttan
c6a8a2d9-eaa1-4963-8c2c-5aed5d7e0503
Cracknell, Victoria, Kathryn Bonnell
3a73f405-4c9f-41c9-83a7-2d49ef0f0313
Yong, Hannah E.J.
9d6a310c-8309-4d94-9dd2-7766cb0420ac
Sharma, Neha
c5c663b2-86e4-4e3c-8f56-c28351419587
Cazenave-Gassiot, Amaury
f53d5438-9fad-4fd6-98f9-3e289205dcd7
Bendt, Anne K.
3a269eb1-2755-4020-a6a1-12100ae89bd4
Godfrey, Keith
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Lewis, Rohan
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Wenk, Markus R.
d1ba356f-b0ed-4318-886a-c8f929679060
Chan, Shiao-Yng
3c9d8970-2cc4-430a-86a7-96f6029a5293

Watkins, Oliver C., Selvam, Preben, Pillai, Reshma Appukuttan, Cracknell, Victoria, Kathryn Bonnell, Yong, Hannah E.J., Sharma, Neha, Cazenave-Gassiot, Amaury, Bendt, Anne K., Godfrey, Keith, Lewis, Rohan, Wenk, Markus R. and Chan, Shiao-Yng (2021) Placental 13C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight. Molecular Medicine. (doi:10.1186/s10020-021-00344-w).

Record type: Article

Abstract

Background: fetal docosahexaenoic acid (DHA) supply relies on preferential transplacental transfer, which is regulated by placental DHA lipid metabolism. Maternal hyperglycemia and obesity associate with higher birthweight and fetal DHA insufficiency but the role of placental DHA metabolism is unclear.

Methods: explants from 17 term placenta were incubated with 13C-labeled DHA for 48h, at 5 or 10 mmol/L glucose treatment, and the production of seventeen individual newly synthesized 13C-DHA labeled lipids quantified by liquid chromatography mass spectrometry.

Results: maternal BMI positively associated with 13C-DHA-labeled diacylglycerols, triacylglycerols, lysophospholipids, phosphatidylcholine and phosphatidylethanolamine plasmalogens, while maternal fasting glycemia positively associated with five 13C-DHA triacylglycerols. In turn, 13C-DHA-labeled phospholipids and triacylglycerols positively associated with birthweight centile. In-vitro glucose treatment increased most 13C-DHA-lipids, but decreased 13C-DHA phosphatidylethanolamine plasmalogens. However, with increasing maternal BMI, the magnitude of the glucose treatment induced increase in 13C-DHA phosphatidylcholine and 13C-DHA lysophospholipids was curtailed, with further decline in 13C-DHA phosphatidylethanolamine plasmalogens. Conversely, with increasing birthweight centile glucose treatment induced increases in 13C-DHA triacylglycerols were exaggerated, while glucose treatment induced decreases in 13C-DHA phosphatidylethanolamine plasmalogens were diminished.

Conclusions: maternal BMI and glycemia increased the production of different placental DHA lipids implying impact on different metabolic pathways. Glucose-induced elevation in placental DHA metabolism is moderated with higher maternal BMI. In turn, findings of associations between many DHA lipids with birthweight suggest that BMI and glycemia promote fetal growth partly through changes in placental DHA metabolism.

Text
Placental 13C DHA metabolism resubmitted_12_7_21 - Accepted Manuscript
Restricted to Repository staff only until 26 August 2022.
Available under License Creative Commons Attribution.
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Accepted/In Press date: 26 July 2021
Published date: 6 August 2021

Identifiers

Local EPrints ID: 450550
URI: http://eprints.soton.ac.uk/id/eprint/450550
ISSN: 1076-1551
PURE UUID: 1cf6a911-27b7-4f83-ab70-a492b153ae9a
ORCID for Keith Godfrey: ORCID iD orcid.org/0000-0002-4643-0618
ORCID for Rohan Lewis: ORCID iD orcid.org/0000-0003-4044-9104

Catalogue record

Date deposited: 03 Aug 2021 16:31
Last modified: 08 Oct 2021 01:37

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Contributors

Author: Oliver C. Watkins
Author: Preben Selvam
Author: Reshma Appukuttan Pillai
Author: Victoria, Kathryn Bonnell Cracknell
Author: Hannah E.J. Yong
Author: Neha Sharma
Author: Amaury Cazenave-Gassiot
Author: Anne K. Bendt
Author: Keith Godfrey ORCID iD
Author: Rohan Lewis ORCID iD
Author: Markus R. Wenk
Author: Shiao-Yng Chan

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