The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Dietary supplementation with transgenic Camelina sativa oil containing 20:5n-3 and 22:6n-3 or fish oil induces differential changes in the transcriptome of CD3+ T lymphocytes

Dietary supplementation with transgenic Camelina sativa oil containing 20:5n-3 and 22:6n-3 or fish oil induces differential changes in the transcriptome of CD3+ T lymphocytes
Dietary supplementation with transgenic Camelina sativa oil containing 20:5n-3 and 22:6n-3 or fish oil induces differential changes in the transcriptome of CD3+ T lymphocytes

Eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) are important for leukocyte function. This study investigated whether consuming transgenic Camelina sativa (tCSO) seed oil containing both 20:5n-3 and 22:6n-3 is as effective as fish oil (FO) for increasing the 20:5n-3 and 22:6n-3 content of leukocytes and altering mitogen-induced changes to the T cell transcriptome. Healthy adults (n = 31) consumed 450 mg/day of 20:5n-3 plus 22:6n-3 from either FO or tCSO for 8 weeks. Blood was collected before and after the intervention. 20:5n-3 and 22:6n-3 incorporation from tCSO into immune cell total lipids was comparable to FO. The relative expression of the transcriptomes of mitogen-stimulated versus unstimulated T lymphocytes in a subgroup of 16 women/test oil showed 4390 transcripts were differentially expressed at Baseline (59% up-regulated), 4769 (57% up-regulated) after FO and 3443 (38% up-regulated) after tCSO supplementation. The 20 most altered transcripts after supplementation differed between test oils. The most altered pathways were associated with cell proliferation and immune function. In conclusion, 20:5n-3 and 22:6n-3 incorporation into immune cells from tCSO was comparable to FO and can modify mitogen-induced changes in the T cell transcriptome, contingent on the lipid matrix of the oil.

Docosahexaenoic acid, Eicosapentaenoic acid, Fish oil, Omega-3, RNAseq, T lymphocyte, Transgenic plant
2072-6643
West, Annette
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Han, Lihua
cd10f8f0-04f0-44ee-a52f-337b4cc8aad8
Lillycrop, Karen
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Napier, Johnathan
b3c688c3-f270-4ebb-b9a7-250011c8a960
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
West, Annette
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Han, Lihua
cd10f8f0-04f0-44ee-a52f-337b4cc8aad8
Lillycrop, Karen
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Napier, Johnathan
b3c688c3-f270-4ebb-b9a7-250011c8a960
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham
09d60a07-8ca1-4351-9bf1-de6ffcfb2159

West, Annette, Miles, Elizabeth, Han, Lihua, Lillycrop, Karen, Napier, Johnathan, Calder, Philip and Burdge, Graham (2021) Dietary supplementation with transgenic Camelina sativa oil containing 20:5n-3 and 22:6n-3 or fish oil induces differential changes in the transcriptome of CD3+ T lymphocytes. Nutrients, 13 (9), [3116]. (doi:10.3390/nu13093116).

Record type: Article

Abstract

Eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) are important for leukocyte function. This study investigated whether consuming transgenic Camelina sativa (tCSO) seed oil containing both 20:5n-3 and 22:6n-3 is as effective as fish oil (FO) for increasing the 20:5n-3 and 22:6n-3 content of leukocytes and altering mitogen-induced changes to the T cell transcriptome. Healthy adults (n = 31) consumed 450 mg/day of 20:5n-3 plus 22:6n-3 from either FO or tCSO for 8 weeks. Blood was collected before and after the intervention. 20:5n-3 and 22:6n-3 incorporation from tCSO into immune cell total lipids was comparable to FO. The relative expression of the transcriptomes of mitogen-stimulated versus unstimulated T lymphocytes in a subgroup of 16 women/test oil showed 4390 transcripts were differentially expressed at Baseline (59% up-regulated), 4769 (57% up-regulated) after FO and 3443 (38% up-regulated) after tCSO supplementation. The 20 most altered transcripts after supplementation differed between test oils. The most altered pathways were associated with cell proliferation and immune function. In conclusion, 20:5n-3 and 22:6n-3 incorporation into immune cells from tCSO was comparable to FO and can modify mitogen-induced changes in the T cell transcriptome, contingent on the lipid matrix of the oil.

Text
- Accepted Manuscript
Available under License Creative Commons Attribution.
Download (1MB)
Text
West et al Nutrients 2021 - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)
Text
Supplementary online material updated - Other
Available under License Creative Commons Attribution.
Download (154kB)

More information

Accepted/In Press date: 2 September 2021
e-pub ahead of print date: 5 September 2021
Published date: 5 September 2021
Keywords: Docosahexaenoic acid, Eicosapentaenoic acid, Fish oil, Omega-3, RNAseq, T lymphocyte, Transgenic plant

Identifiers

Local EPrints ID: 451019
URI: http://eprints.soton.ac.uk/id/eprint/451019
ISSN: 2072-6643
PURE UUID: 4e26a97a-a611-49e5-b77c-22fdb61fda60
ORCID for Elizabeth Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Karen Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X
ORCID for Graham Burdge: ORCID iD orcid.org/0000-0002-7665-2967

Catalogue record

Date deposited: 03 Sep 2021 16:30
Last modified: 26 Nov 2021 02:38

Export record

Altmetrics

Contributors

Author: Annette West
Author: Elizabeth Miles ORCID iD
Author: Lihua Han
Author: Karen Lillycrop ORCID iD
Author: Johnathan Napier
Author: Philip Calder ORCID iD
Author: Graham Burdge ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×