An in vitro biofilm model system to facilitate study of microbial communities of the human oral cavity
An in vitro biofilm model system to facilitate study of microbial communities of the human oral cavity
The human oral cavity is host to a diverse microbiota. Much of what is known about the behaviour of oral microbes derives from studies of individual or several cultivated species, situations which do not totally reflect the function of organisms within more complex microbiota or multispecies biofilms. The number of validated models that allow examination of the role that biofilms play during oral cavity colonization is also limited. The CDC biofilm reactor is a standard method that has been deployed to study interactions between members of human microbiotas allowing studies to be completed during an extended period under conditions where nutrient availability, and washout of waste products are controlled. The objective of this work was to develop a robust in vitro biofilm-model system from a pooled saliva inoculum to study the development, reproducibility and stability of the oral microbiota. By employing deep sequencing of the variable regions of the 16S rRNA gene, we found that the CDC biofilm reactor could be used to efficiently cultivate microbiota containing all six major phyla previously identified as the core saliva microbiota. After an acclimatisation period, communities in each reactor stabilised. Replicate reactors were predominately populated by a shared core microbiota; variation between replicate reactors was primarily driven by shifts in abundance of shared operational taxonomic units. We conclude that the CDC biofilm reactor can be used to cultivate communities that replicate key features of the human oral cavity and is a useful tool to facilitate studies of the dynamics of these communities.
302-310
An, Shi-Qi
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Hull, Robert
62071424-f43f-4eeb-bd04-7910b7fcfa9a
Métris, Aline
4b122c22-8489-4dee-859e-f1867a22bbda
Barrett, Paul
d6e6d76e-0352-4be2-a0ae-302a2cf54c4e
Webb, Jeremy
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
26 November 2021
An, Shi-Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Hull, Robert
62071424-f43f-4eeb-bd04-7910b7fcfa9a
Métris, Aline
4b122c22-8489-4dee-859e-f1867a22bbda
Barrett, Paul
d6e6d76e-0352-4be2-a0ae-302a2cf54c4e
Webb, Jeremy
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
An, Shi-Qi, Hull, Robert, Métris, Aline, Barrett, Paul, Webb, Jeremy and Stoodley, Paul
(2021)
An in vitro biofilm model system to facilitate study of microbial communities of the human oral cavity.
Letters in Applied Microbiology, 74 (3), .
(doi:10.1111/lam.13618).
Abstract
The human oral cavity is host to a diverse microbiota. Much of what is known about the behaviour of oral microbes derives from studies of individual or several cultivated species, situations which do not totally reflect the function of organisms within more complex microbiota or multispecies biofilms. The number of validated models that allow examination of the role that biofilms play during oral cavity colonization is also limited. The CDC biofilm reactor is a standard method that has been deployed to study interactions between members of human microbiotas allowing studies to be completed during an extended period under conditions where nutrient availability, and washout of waste products are controlled. The objective of this work was to develop a robust in vitro biofilm-model system from a pooled saliva inoculum to study the development, reproducibility and stability of the oral microbiota. By employing deep sequencing of the variable regions of the 16S rRNA gene, we found that the CDC biofilm reactor could be used to efficiently cultivate microbiota containing all six major phyla previously identified as the core saliva microbiota. After an acclimatisation period, communities in each reactor stabilised. Replicate reactors were predominately populated by a shared core microbiota; variation between replicate reactors was primarily driven by shifts in abundance of shared operational taxonomic units. We conclude that the CDC biofilm reactor can be used to cultivate communities that replicate key features of the human oral cavity and is a useful tool to facilitate studies of the dynamics of these communities.
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Manuscript final 19Sept2021
- Accepted Manuscript
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Accepted/In Press date: 26 October 2021
Published date: 26 November 2021
Additional Information:
Funding Information:
This work was funded by Unilever and supported in part by grants from the BBSRC (BB/R012415/1 to JSW), National Biofilms Innovation Centre Proof of Concept Grant (NBIC 01POC18035 to PS, JSW).
Publisher Copyright:
© 2021 The Authors. Letters in Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.
Identifiers
Local EPrints ID: 452229
URI: http://eprints.soton.ac.uk/id/eprint/452229
ISSN: 0266-8254
PURE UUID: 57af162e-4f1d-4a7e-ab91-02957165b82c
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Date deposited: 30 Nov 2021 17:33
Last modified: 17 Mar 2024 06:56
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Author:
Shi-Qi An
Author:
Robert Hull
Author:
Aline Métris
Author:
Paul Barrett
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