Mapping bacterial biofilm on features of orthopedic implants in vitro
Mapping bacterial biofilm on features of orthopedic implants in vitro
Implant-associated infection is a major complication of orthopedic surgery. One of the most common organisms identified in periprosthetic joint infections is Staphylococcus aureus, a biofilmforming pathogen. Orthopedic implants are composed of a variety of materials, such as titanium, polyethylene and stainless steel, which are at risk for colonization by bacterial biofilms. Little is known about how larger surface features of orthopedic hardware (such as ridges, holes, edges, etc.) influence biofilm formation and attachment. To study how biofilms might form on actual components, we submerged multiple orthopedic implants of various shapes, sizes, roughness and material type in brain heart infusion broth inoculated with Staphylococcus aureus SAP231, a bioluminescent USA300 strain. Implants were incubated for 72 h with daily media exchanges. After incubation, implants were imaged using an in vitro imaging system (IVIS) and the metabolic signal produced by biofilms was quantified by image analysis. Scanning electron microscopy was then used to image different areas of the implants to complement the IVIS imaging. Rough surfaces had the greatest luminescence compared to edges or smooth surfaces on a single implant and across all implants when the images were merged. The luminescence of edges was also significantly greater than smooth surfaces. These data suggest implant roughness, as well as large-scale surface features, may be at greater risk of biofilm colonization.
biofilm attachment, biofilm mapping, implant-associated infection, orthopedic biomaterials, periprosthetic joint infection, surface roughness, Implant-associated infection, Orthopedic biomaterials, Periprosthetic joint infection, Biofilm attachment, Biofilm mapping, Surface roughness
586
Moore, Kelly
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Gupta, Niraj K.
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Gupta, Tripti Thapa
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Patel, Khushi
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Brooks, Jacob R.
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Sullivan, Anne
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Litsky, Alan S.
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Stoodley, Paul
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8 March 2022
Moore, Kelly
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Gupta, Niraj K.
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Gupta, Tripti Thapa
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Patel, Khushi
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Brooks, Jacob R.
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Sullivan, Anne
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Litsky, Alan S.
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Stoodley, Paul
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Moore, Kelly, Gupta, Niraj K., Gupta, Tripti Thapa, Patel, Khushi, Brooks, Jacob R., Sullivan, Anne, Litsky, Alan S. and Stoodley, Paul
(2022)
Mapping bacterial biofilm on features of orthopedic implants in vitro.
Microorganisms, 10 (3), , [586].
(doi:10.3390/microorganisms10030586).
Abstract
Implant-associated infection is a major complication of orthopedic surgery. One of the most common organisms identified in periprosthetic joint infections is Staphylococcus aureus, a biofilmforming pathogen. Orthopedic implants are composed of a variety of materials, such as titanium, polyethylene and stainless steel, which are at risk for colonization by bacterial biofilms. Little is known about how larger surface features of orthopedic hardware (such as ridges, holes, edges, etc.) influence biofilm formation and attachment. To study how biofilms might form on actual components, we submerged multiple orthopedic implants of various shapes, sizes, roughness and material type in brain heart infusion broth inoculated with Staphylococcus aureus SAP231, a bioluminescent USA300 strain. Implants were incubated for 72 h with daily media exchanges. After incubation, implants were imaged using an in vitro imaging system (IVIS) and the metabolic signal produced by biofilms was quantified by image analysis. Scanning electron microscopy was then used to image different areas of the implants to complement the IVIS imaging. Rough surfaces had the greatest luminescence compared to edges or smooth surfaces on a single implant and across all implants when the images were merged. The luminescence of edges was also significantly greater than smooth surfaces. These data suggest implant roughness, as well as large-scale surface features, may be at greater risk of biofilm colonization.
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microorganisms-1517810 revision final
- Accepted Manuscript
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microorganisms-10-00586
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More information
Accepted/In Press date: 5 March 2022
e-pub ahead of print date: 8 March 2022
Published date: 8 March 2022
Additional Information:
Funding Information:
We acknowledge resources from the Center for Electron Microscopy and Analysis (CEMAS) and Nanotech West Lab at The Ohio State University. We thank Dr Maurice Manring of the OSU Orthopaedic Dept. for help with the editing and submission.
Funding Information:
Funding: This research was funded by NIH grant R01GM124436 (P.S.).
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords:
biofilm attachment, biofilm mapping, implant-associated infection, orthopedic biomaterials, periprosthetic joint infection, surface roughness, Implant-associated infection, Orthopedic biomaterials, Periprosthetic joint infection, Biofilm attachment, Biofilm mapping, Surface roughness
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Local EPrints ID: 455792
URI: http://eprints.soton.ac.uk/id/eprint/455792
PURE UUID: 37f397c3-a41e-4721-9613-2cc2fa63109d
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Date deposited: 04 Apr 2022 16:48
Last modified: 17 Mar 2024 03:18
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Contributors
Author:
Kelly Moore
Author:
Niraj K. Gupta
Author:
Tripti Thapa Gupta
Author:
Khushi Patel
Author:
Jacob R. Brooks
Author:
Anne Sullivan
Author:
Alan S. Litsky
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