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Development of a multi-parameter in vivo sensing platform for intrauterine studies and subfertility diagnostics

Development of a multi-parameter in vivo sensing platform for intrauterine studies and subfertility diagnostics
Development of a multi-parameter in vivo sensing platform for intrauterine studies and subfertility diagnostics
Couples in the developed world are increasingly being confronted with subfertility issues. In over 18% of the cases no clear reason can be identified. It is suspected that abnormalities in the physiological parameters of the uterine environment have a significant influence on embryo implantation and development. However, data from human patients is highly limited and has not been revisited for decades. Empirical studies on in vitro fertilisation (IVF) have already confirmed that levels of pH and dissolved oxygen (DO) can inhibit embryo development and decrease implantation success rates. As the current success rate for IVF remains stagnant at 33%, information on the intrauterine environment can be of great importance. Hitherto, diagnostic capabilities to assess these parameters in vivo are still lacking. This thesis describes the development of a system to monitor levels of pH and DO in utero. A pH and DO sensor together with a miniature reference electrode (RE) are incorporated onto a single, double sided sensor die sized 2.3 x 4.3 mm. The performance of the reference electrode and the individual sensors has been assessed to determine their applicability in an implantable system with power constraints, measuring unobtrusively for a period of 30 days. It is key that the biological relevancy is not compromised by sensor characteristics such as accuracy, precision and long-term drift. Both pH and DO sensors used a miniature silver silver chloride (Ag-AgCl) reference electrode, formed through the electrochemical deposition of Silver onto Platinum electrodes. Excellent performance has been observed over a 40 day period with a yield of 90% by employing a two day preliminary test. The use of an internal biocompatible electrolyte has been investigated, developed and tested. Although no added advantage was noted the described theory, simulation and results have led to an integrated sensor package developed in parallel with the sensors.
University of Southampton
Mingels, Roeland
4acacc8b-913a-4eaf-b683-4287a2f784a3
Mingels, Roeland
4acacc8b-913a-4eaf-b683-4287a2f784a3
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Mingels, Roeland (2018) Development of a multi-parameter in vivo sensing platform for intrauterine studies and subfertility diagnostics. University of Southampton, Doctoral Thesis, 175pp.

Record type: Thesis (Doctoral)

Abstract

Couples in the developed world are increasingly being confronted with subfertility issues. In over 18% of the cases no clear reason can be identified. It is suspected that abnormalities in the physiological parameters of the uterine environment have a significant influence on embryo implantation and development. However, data from human patients is highly limited and has not been revisited for decades. Empirical studies on in vitro fertilisation (IVF) have already confirmed that levels of pH and dissolved oxygen (DO) can inhibit embryo development and decrease implantation success rates. As the current success rate for IVF remains stagnant at 33%, information on the intrauterine environment can be of great importance. Hitherto, diagnostic capabilities to assess these parameters in vivo are still lacking. This thesis describes the development of a system to monitor levels of pH and DO in utero. A pH and DO sensor together with a miniature reference electrode (RE) are incorporated onto a single, double sided sensor die sized 2.3 x 4.3 mm. The performance of the reference electrode and the individual sensors has been assessed to determine their applicability in an implantable system with power constraints, measuring unobtrusively for a period of 30 days. It is key that the biological relevancy is not compromised by sensor characteristics such as accuracy, precision and long-term drift. Both pH and DO sensors used a miniature silver silver chloride (Ag-AgCl) reference electrode, formed through the electrochemical deposition of Silver onto Platinum electrodes. Excellent performance has been observed over a 40 day period with a yield of 90% by employing a two day preliminary test. The use of an internal biocompatible electrolyte has been investigated, developed and tested. Although no added advantage was noted the described theory, simulation and results have led to an integrated sensor package developed in parallel with the sensors.

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Published date: May 2018

Identifiers

Local EPrints ID: 455939
URI: http://eprints.soton.ac.uk/id/eprint/455939
PURE UUID: 805ecfe5-c830-4d5c-a01d-9e90961fe9d5
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

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Date deposited: 08 Apr 2022 17:55
Last modified: 09 Apr 2022 04:01

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Contributors

Author: Roeland Mingels
Thesis advisor: Hywel Morgan ORCID iD

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