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A commercial SnF2 toothpaste formulation reduces simulated human plaque biofilm in a dynamic typodont model

A commercial SnF2 toothpaste formulation reduces simulated human plaque biofilm in a dynamic typodont model
A commercial SnF2 toothpaste formulation reduces simulated human plaque biofilm in a dynamic typodont model
Aims: we present a dynamic typodont biofilm model (DTBM) incorporating 1) human dentition anatomy, 2) fluid flow over intermittently fluid bathed tooth surfaces and 3) an oxic headspace to allow aerobic and anaerobic niches to develop naturally, as a screening tool to assess the effect of stannous fluoride (SnF2) toothpaste against a simulated human plaque biofilm (SPB).

Methods and results: first, hydroxyapatite (HA) coupons were inoculated with human saliva/plaque and cultured at 37oC under air. Selected species representative of common commensal and anaerobic pathogens were quantified for relative abundance changes over 4d by PCR densitometry to confirm the culture conditions allowed the proliferation of these species. A continuous culture DTBM reactor on a rocker table was inoculated with saliva/plaque and incubated at 37°C for 24h. Tooth shear stress was estimated by particle tracking. A SnF2 toothpaste solution, or a sham rise was administered twice daily for 3d to mimic routine oral hygiene. SPB biomass was assessed by total bacterial DNA and methylene blue (MB) staining. Early colonizer aerobes and late colonizer anaerobes species were detected in the HA and DTBM, and the trends in changing abundance were consistent with those seen clinically.

Conclusions: treatment with the SnF2 solution showed significant reductions of 53.05% and 54.4% in the SPB by MB staining and DNA, respectively. Significance and impact of study: The model has potential for assessing dentition anatomy and fluid flow on the efficacy of antimicrobial efficacy against localized SPB and may be amenable to the plaque index clinical evaluation.
Dental, biofilm, dentition model, stannous fluoride
1364-5072
1341-1352
Khosravi, Yalda
e341065c-78aa-4780-9234-7e5f3cbe850f
Palmer, Sara
05ad99fd-a12f-41e5-9b43-e6e0d22a55ca
Amorin Daep, Carlo
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Sambanthamoorthy, Karthik
e78c92eb-9ff8-4fd3-9ab2-2d52f7084d0b
Kumar, Purnima
24a05e97-0489-4a30-a3eb-dac29ecc2d1c
Dusane, Devendra H.
9a47c5eb-5587-4f1d-bfd4-8548681be2bc
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Khosravi, Yalda
e341065c-78aa-4780-9234-7e5f3cbe850f
Palmer, Sara
05ad99fd-a12f-41e5-9b43-e6e0d22a55ca
Amorin Daep, Carlo
91743b1f-721a-4e3c-9ead-e900c5870b81
Sambanthamoorthy, Karthik
e78c92eb-9ff8-4fd3-9ab2-2d52f7084d0b
Kumar, Purnima
24a05e97-0489-4a30-a3eb-dac29ecc2d1c
Dusane, Devendra H.
9a47c5eb-5587-4f1d-bfd4-8548681be2bc
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f

Khosravi, Yalda, Palmer, Sara, Amorin Daep, Carlo, Sambanthamoorthy, Karthik, Kumar, Purnima, Dusane, Devendra H. and Stoodley, Paul (2022) A commercial SnF2 toothpaste formulation reduces simulated human plaque biofilm in a dynamic typodont model. Journal of Applied Microbiology, 133 (3), 1341-1352. (doi:10.1111/jam.15634).

Record type: Article

Abstract

Aims: we present a dynamic typodont biofilm model (DTBM) incorporating 1) human dentition anatomy, 2) fluid flow over intermittently fluid bathed tooth surfaces and 3) an oxic headspace to allow aerobic and anaerobic niches to develop naturally, as a screening tool to assess the effect of stannous fluoride (SnF2) toothpaste against a simulated human plaque biofilm (SPB).

Methods and results: first, hydroxyapatite (HA) coupons were inoculated with human saliva/plaque and cultured at 37oC under air. Selected species representative of common commensal and anaerobic pathogens were quantified for relative abundance changes over 4d by PCR densitometry to confirm the culture conditions allowed the proliferation of these species. A continuous culture DTBM reactor on a rocker table was inoculated with saliva/plaque and incubated at 37°C for 24h. Tooth shear stress was estimated by particle tracking. A SnF2 toothpaste solution, or a sham rise was administered twice daily for 3d to mimic routine oral hygiene. SPB biomass was assessed by total bacterial DNA and methylene blue (MB) staining. Early colonizer aerobes and late colonizer anaerobes species were detected in the HA and DTBM, and the trends in changing abundance were consistent with those seen clinically.

Conclusions: treatment with the SnF2 solution showed significant reductions of 53.05% and 54.4% in the SPB by MB staining and DNA, respectively. Significance and impact of study: The model has potential for assessing dentition anatomy and fluid flow on the efficacy of antimicrobial efficacy against localized SPB and may be amenable to the plaque index clinical evaluation.

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More information

Accepted/In Press date: 23 May 2022
e-pub ahead of print date: 23 May 2022
Published date: 1 September 2022
Additional Information: Funding Information: This project was funded by Colgate‐Palmolive (PS). Funding Information: PS is funded by Colgate‐Palmolive. CAD and KS helped design the study. Colgate‐Palmolive provided Colgate Total SnF in a blinded unmarked toothpaste tube. Publisher Copyright: © 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.
Keywords: Dental, biofilm, dentition model, stannous fluoride

Identifiers

Local EPrints ID: 457887
URI: http://eprints.soton.ac.uk/id/eprint/457887
ISSN: 1364-5072
PURE UUID: f0524d66-c453-462c-91b2-58f344cb83d2
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

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Date deposited: 21 Jun 2022 18:13
Last modified: 17 Mar 2024 03:18

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Contributors

Author: Yalda Khosravi
Author: Sara Palmer
Author: Carlo Amorin Daep
Author: Karthik Sambanthamoorthy
Author: Purnima Kumar
Author: Devendra H. Dusane
Author: Paul Stoodley ORCID iD

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