Synthesis and application of polystyrene latex microspheres
Synthesis and application of polystyrene latex microspheres
The aims of this project were the synthesis of polystyrene latex microspheres, within a size range capable of being able to be taken up by cells, and their subsequent functionalization with biomolecules, such as oligonucleotides or oligopeptides. Polystyrene latex microspheres possessing hydroxy, carboxylate and amino functionalities were synthesized by emulsifier-free emulsion co-polymerization. The polystyrene latex microspheres were characterized by IR and titration methods. The size distribution was determined by scanning electron microscopy and photon correlation spectroscopy. The synthesized microspheres were labelled with dyes such as 4(5)- carboxyfluorescein and studies with ND7 cells indicated cellular uptake of these microspheres, offering potential for development as a reporter or carrier system. Successful solution phase studies with microsphere bound olgionucleotides and a FRET peptide showed the possibility of use as a real time probe.
University of Southampton
Chitkul, Nutcha
c77e8d2d-2d6c-4776-a42c-9fae5da1d36d
2002
Chitkul, Nutcha
c77e8d2d-2d6c-4776-a42c-9fae5da1d36d
Chitkul, Nutcha
(2002)
Synthesis and application of polystyrene latex microspheres.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The aims of this project were the synthesis of polystyrene latex microspheres, within a size range capable of being able to be taken up by cells, and their subsequent functionalization with biomolecules, such as oligonucleotides or oligopeptides. Polystyrene latex microspheres possessing hydroxy, carboxylate and amino functionalities were synthesized by emulsifier-free emulsion co-polymerization. The polystyrene latex microspheres were characterized by IR and titration methods. The size distribution was determined by scanning electron microscopy and photon correlation spectroscopy. The synthesized microspheres were labelled with dyes such as 4(5)- carboxyfluorescein and studies with ND7 cells indicated cellular uptake of these microspheres, offering potential for development as a reporter or carrier system. Successful solution phase studies with microsphere bound olgionucleotides and a FRET peptide showed the possibility of use as a real time probe.
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Published date: 2002
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Local EPrints ID: 464902
URI: http://eprints.soton.ac.uk/id/eprint/464902
PURE UUID: d0be98f5-2be2-456a-93d0-0410d0c94d58
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Date deposited: 05 Jul 2022 00:08
Last modified: 16 Mar 2024 19:49
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Author:
Nutcha Chitkul
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