A high-resolution map of human RNA translation

Accepted/In Press date: 15 June 2022

e-pub ahead of print date: 15 July 2022

Published date: 4 August 2022

Additional Information: Funding Information: We thank Dr. Dennis Kappei for providing expert feedback on proteomics analysis. This work was supported by the Open Fund -Young Individual Research Grant (OFYIRG18nov-0014), Duke-NUS-GCR/2018/0017A Grant, and the Academic Clinical Programme Charles Toh Cardiovascular Fellowship Award to S. Schafer. The work was also supported by a Singapore National Research Foundation (NRF) Competitive Research Programme (CRP) grant (NRF-CRP20-2017-0002) and National Medical Research Council (NMRC) Young Investigators Research Grant (YIRG) (NMRC/OFYIRG/0022/2016) awarded to O. Rackham. L.H. is supported by NRF-NRFF2017-05 and HHMI IRSP 55008732. O.J.L.R. S.A.C. and S.S. conceived and arranged funding for the study. O.J.L.R. and S.S. designed and managed the implementation of the study. S.P.C. E.A. O.J.L.R. and S.S. wrote the manuscript with input from coauthors. S.P.C. performed data processing, Ribo-seq analysis, and pipeline development and implementation. E.A. performed and organized the molecular biology experiments. S.P.C. and E.A. created the visualization of results. S.L. A.A.W. S.V. W.M. and W.W.L. assisted in molecular biology experiments and in vitro cell culture. S.R.L. and N.T.Z. assisted with mass spec analysis. G.D. J.F.O. and N.H. performed aspects of the data processing and N.H. provided guidance for the evolutionary analysis. N.W. assisted in the manuscript preparation and genetic aspects of the Ribo-seq analysis. C.J.P. assisted with sequencing. J.M.P. C.Q.E.L. A.G. J.C. J.P.K. and K.T. provided samples collection and expertise in cell culture. L.H. provided in-depth feedback during the manuscript preparation and provided expertise in smORF detection. Having provided an equal contribution toward this work, the first and senior authors are listed in an arbitrary order. S.S. and S.A.C. are co-founders and shareholders of Enleofen Bio Pte Ltd. O.J.L.R. and J.M.P. are SAB members and shareholders of Mogrify Ltd. All other authors declare no competing interests. Funding Information: We thank Dr. Dennis Kappei for providing expert feedback on proteomics analysis. This work was supported by the Open Fund -Young Individual Research Grant ( OFYIRG18nov-0014 ), Duke-NUS-GCR/2018/0017A Grant, and the Academic Clinical Programme Charles Toh Cardiovascular Fellowship Award to S. Schafer. The work was also supported by a Singapore National Research Foundation (NRF) Competitive Research Programme (CRP) grant ( NRF-CRP20-2017-0002 ) and National Medical Research Council (NMRC) Young Investigators Research Grant (YIRG) ( NMRC/OFYIRG/0022/2016 ) awarded to O. Rackham. L.H. is supported by NRF-NRFF2017-05 and HHMI IRSP 55008732 . Publisher Copyright: © 2022 Elsevier Inc.

Related URLs:

• https://linkinghub.elsevier.co...6522006062

Keywords: Ribo-seq, Ribosome profiling, dORFs, lncRNAs, smORFs, translation, uORFs, untranslated regions

Local EPrints ID: 469988

URI: http://eprints.soton.ac.uk/id/eprint/469988

DOI: doi:10.1016/j.molcel.2022.06.023

ISSN: 1097-2765

PURE UUID: c25ad0ae-0cf0-41ff-9101-8038f1ae8e34

ORCID for Owen J.l. Rackham: orcid.org/0000-0002-4390-0872

Date deposited: 29 Sep 2022 16:48

Last modified: 17 Mar 2024 07:29