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An exploratory study provides insights into MMP9 and Aβ levels in the vitreous and blood across different ages and in a subset of AMD patients

An exploratory study provides insights into MMP9 and Aβ levels in the vitreous and blood across different ages and in a subset of AMD patients
An exploratory study provides insights into MMP9 and Aβ levels in the vitreous and blood across different ages and in a subset of AMD patients
Matrix metalloproteinase-9 (MMP9) and total amyloid-beta (Aβ) are prospective biomarkers of ocular ageing and retinopathy. These were quantified by ELISA in the vitreous and blood from controls (n = 55) and in a subset of age-related macular degeneration (AMD) patients (n = 12) for insights and possible additional links between the ocular and systemic compartments. Vitreous MMP9 levels in control and AMD groups were 932.5 ± 240.9 pg/mL and 813.7 ± 157.6 pg/mL, whilst serum levels were 2228 ± 193 pg/mL and 2386.8 ± 449.4 pg/mL, respectively. Vitreous Aβ in control and AMD groups were 1173.5 ± 117.1 pg/mL and 1275.6 ± 332.9 pg/mL, whilst plasma Aβ were 574.3 ± 104.8 pg/mL and 542.2 ± 139.9 pg/mL, respectively. MMP9 and Aβ showed variable levels across the lifecourse, indicating no correlation to each other or with age nor AMD status, though the smaller AMD cohort was a limiting factor. Aβ and MMP9 levels in the vitreous and blood were unrelated to mean arterial pressure. Smoking, another modifiable risk, showed no association with vitreous Aβ. However, smoking may be linked with vitreous (p = 0.004) and serum (p = 0.005) MMP9 levels in control and AMD groups, though this did not reach our elevated (p = 0.001) significance. A bioinformatics analysis revealed promising MMP9 and APP/Aβ partners for further scrutiny, many of which are already linked with retinopathy.
MMP, amyloid beta (Aβ), age-related macular degeneration (AMD), biomarkers, lifecourse, lifestyle, smoking, mean arterial pressure
1661-6596
1
Lynn, Savannah
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Soubigou, Flavie
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Dewing, Jennifer
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Smith, Amanda
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Ballingall, Joanna
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Cree, Angela
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Sass, Thea
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Nica, Isabela
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Watkins, Catrin
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Gupta, Bashar
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Almuhtaseb, Hussein
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Lash, Stephen C.
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Yuen, Ho Ming
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Cree, Angela
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Newman, Tracey
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Lotery, Andrew
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Ratnayaka, J. Arjuna
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Lynn, Savannah
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Soubigou, Flavie
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Dewing, Jennifer
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Smith, Amanda
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Ballingall, Joanna
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Cree, Angela
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Sass, Thea
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Nica, Isabela
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Watkins, Catrin
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Gupta, Bashar
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Almuhtaseb, Hussein
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Lash, Stephen C.
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Yuen, Ho Ming
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Cree, Angela
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Newman, Tracey
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Lotery, Andrew
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Ratnayaka, J. Arjuna
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Lynn, Savannah, Soubigou, Flavie, Dewing, Jennifer, Smith, Amanda, Ballingall, Joanna, Cree, Angela, Sass, Thea, Nica, Isabela, Watkins, Catrin, Gupta, Bashar, Almuhtaseb, Hussein, Lash, Stephen C., Yuen, Ho Ming, Cree, Angela, Newman, Tracey, Lotery, Andrew and Ratnayaka, J. Arjuna (2022) An exploratory study provides insights into MMP9 and Aβ levels in the vitreous and blood across different ages and in a subset of AMD patients. International Journal of Molecular Sciences, 1. (doi:10.3390/ijms232314603.).

Record type: Article

Abstract

Matrix metalloproteinase-9 (MMP9) and total amyloid-beta (Aβ) are prospective biomarkers of ocular ageing and retinopathy. These were quantified by ELISA in the vitreous and blood from controls (n = 55) and in a subset of age-related macular degeneration (AMD) patients (n = 12) for insights and possible additional links between the ocular and systemic compartments. Vitreous MMP9 levels in control and AMD groups were 932.5 ± 240.9 pg/mL and 813.7 ± 157.6 pg/mL, whilst serum levels were 2228 ± 193 pg/mL and 2386.8 ± 449.4 pg/mL, respectively. Vitreous Aβ in control and AMD groups were 1173.5 ± 117.1 pg/mL and 1275.6 ± 332.9 pg/mL, whilst plasma Aβ were 574.3 ± 104.8 pg/mL and 542.2 ± 139.9 pg/mL, respectively. MMP9 and Aβ showed variable levels across the lifecourse, indicating no correlation to each other or with age nor AMD status, though the smaller AMD cohort was a limiting factor. Aβ and MMP9 levels in the vitreous and blood were unrelated to mean arterial pressure. Smoking, another modifiable risk, showed no association with vitreous Aβ. However, smoking may be linked with vitreous (p = 0.004) and serum (p = 0.005) MMP9 levels in control and AMD groups, though this did not reach our elevated (p = 0.001) significance. A bioinformatics analysis revealed promising MMP9 and APP/Aβ partners for further scrutiny, many of which are already linked with retinopathy.

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Accepted/In Press date: 18 November 2022
Published date: 23 November 2022
Keywords: MMP, amyloid beta (Aβ), age-related macular degeneration (AMD), biomarkers, lifecourse, lifestyle, smoking, mean arterial pressure

Identifiers

Local EPrints ID: 473501
URI: http://eprints.soton.ac.uk/id/eprint/473501
ISSN: 1661-6596
PURE UUID: 63a73a7d-b863-4ea8-9072-2d71f79aab49
ORCID for Savannah Lynn: ORCID iD orcid.org/0000-0003-2513-3144
ORCID for Angela Cree: ORCID iD orcid.org/0000-0002-1987-8900
ORCID for Tracey Newman: ORCID iD orcid.org/0000-0002-3727-9258
ORCID for Andrew Lotery: ORCID iD orcid.org/0000-0001-5541-4305
ORCID for J. Arjuna Ratnayaka: ORCID iD orcid.org/0000-0002-1027-6938

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Date deposited: 20 Jan 2023 17:59
Last modified: 06 Jun 2024 02:03

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Contributors

Author: Savannah Lynn ORCID iD
Author: Flavie Soubigou
Author: Jennifer Dewing
Author: Amanda Smith
Author: Joanna Ballingall
Author: Angela Cree ORCID iD
Author: Thea Sass
Author: Isabela Nica
Author: Catrin Watkins
Author: Bashar Gupta
Author: Hussein Almuhtaseb
Author: Stephen C. Lash
Author: Ho Ming Yuen
Author: Angela Cree
Author: Tracey Newman ORCID iD
Author: Andrew Lotery ORCID iD

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