Influence of dietary n-3 long chain polyunsaturated fatty acid intake on oxylipins in erythrocytes of women with rheumatoid arthritis
Influence of dietary n-3 long chain polyunsaturated fatty acid intake on oxylipins in erythrocytes of women with rheumatoid arthritis
Oxylipins derived from n-3 fatty acids are suggested as the link between these fatty acids and reduced inflammation. The aim of the present study was to explore the effect of a randomized controlled cross-over intervention on oxylipin patterns in erythrocytes. Twenty-three women with rheumatoid arthritis completed 2 × 11-weeks exchanging one cooked meal per day, 5 days a week, for a meal including 75 g blue mussels (source for n-3 fatty acids) or 75 g meat. Erythrocyte oxylipins were quantified by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The results were analyzed with multivariate data analysis. Orthogonal projections to latent structures (OPLS) with effect projections and with discriminant analysis were performed to compare the two diets’ effects on oxylipins. Wilcoxon signed rank test was used to test pre and post values for each dietary period as well as post blue-mussel vs. post meat. The blue-mussel diet led to significant changes in a few oxylipins from the precursor fatty acids arachidonic acid and dihomo-ɣ-linolenic acid. Despite significant changes in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and free EPA in erythrocytes in the mussel group, no concurrent changes in their oxylipins were seen. Further research is needed to study the link between n-3 fatty-acid intake, blood oxylipins, and inflammation.
eicosanoids, erythrocytes, fatty acids, n-3 LC PUFA, oxylipins, rheumatoid arthritis, seafood
Lindqvist, Helen M
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Winkvist, Anna
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Gjertsson, Inger
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Calder, Philip
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Armando, Aaron M
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Quehen-berger, Oswald
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Coras, Roxana
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Guma, Monica
042afc71-5af6-4a07-8b4c-7d59f1d553d5
11 January 2023
Lindqvist, Helen M
95a2f5df-1a2e-4f8e-a338-0f65b3e59cba
Winkvist, Anna
332a155d-d182-4097-be2a-0bf01beca998
Gjertsson, Inger
d60ce40d-cff4-412a-aa47-e95170b7ede9
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Armando, Aaron M
0dde0efa-8f0e-478e-900f-b1db5d078f34
Quehen-berger, Oswald
11df1e7b-f95a-402e-bf16-1faab1f93115
Coras, Roxana
e3b9c71f-3a6d-47a2-b5f4-ba9ec9e734ab
Guma, Monica
042afc71-5af6-4a07-8b4c-7d59f1d553d5
Lindqvist, Helen M, Winkvist, Anna, Gjertsson, Inger, Calder, Philip, Armando, Aaron M, Quehen-berger, Oswald, Coras, Roxana and Guma, Monica
(2023)
Influence of dietary n-3 long chain polyunsaturated fatty acid intake on oxylipins in erythrocytes of women with rheumatoid arthritis.
Molecules, 28 (2), [717].
(doi:10.3390/molecules28020717).
Abstract
Oxylipins derived from n-3 fatty acids are suggested as the link between these fatty acids and reduced inflammation. The aim of the present study was to explore the effect of a randomized controlled cross-over intervention on oxylipin patterns in erythrocytes. Twenty-three women with rheumatoid arthritis completed 2 × 11-weeks exchanging one cooked meal per day, 5 days a week, for a meal including 75 g blue mussels (source for n-3 fatty acids) or 75 g meat. Erythrocyte oxylipins were quantified by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The results were analyzed with multivariate data analysis. Orthogonal projections to latent structures (OPLS) with effect projections and with discriminant analysis were performed to compare the two diets’ effects on oxylipins. Wilcoxon signed rank test was used to test pre and post values for each dietary period as well as post blue-mussel vs. post meat. The blue-mussel diet led to significant changes in a few oxylipins from the precursor fatty acids arachidonic acid and dihomo-ɣ-linolenic acid. Despite significant changes in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and free EPA in erythrocytes in the mussel group, no concurrent changes in their oxylipins were seen. Further research is needed to study the link between n-3 fatty-acid intake, blood oxylipins, and inflammation.
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molecules-28-00717-v2
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Accepted/In Press date: 10 January 2023
Published date: 11 January 2023
Additional Information:
Funding Information:
This research was funded by the Hakansson Foundation (2014) and The Swedish government under the ALF-funds (an agreement between healthcare and research) (Grant Number ALFGBG-716341). Analysis was funded by IRIS-scholarship (2016), Langmanska Cultural Fund 2017 (BA17-0928) and 2020 (BA20-0711) and the Magnus Bergvalls Foundation 2020 (2020-03869). Roxana Coras and Monica Guma were supported by the Krupp Endowed Fund. None of the financial supporters had any role in the design or execution of the study or later analyses of data.
Publisher Copyright:
© 2023 by the authors.
Keywords:
eicosanoids, erythrocytes, fatty acids, n-3 LC PUFA, oxylipins, rheumatoid arthritis, seafood
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Local EPrints ID: 474352
URI: http://eprints.soton.ac.uk/id/eprint/474352
ISSN: 1420-3049
PURE UUID: 58c5f4e7-75f7-4260-861f-4eee9983ba90
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Date deposited: 20 Feb 2023 18:09
Last modified: 17 Mar 2024 02:42
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Author:
Helen M Lindqvist
Author:
Anna Winkvist
Author:
Inger Gjertsson
Author:
Aaron M Armando
Author:
Oswald Quehen-berger
Author:
Roxana Coras
Author:
Monica Guma
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