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Tri-lineage differentiation potential of osteosarcoma cell lines and human bone marrow stromal cells from different anatomical locations

Tri-lineage differentiation potential of osteosarcoma cell lines and human bone marrow stromal cells from different anatomical locations
Tri-lineage differentiation potential of osteosarcoma cell lines and human bone marrow stromal cells from different anatomical locations
The bone cancer osteosarcoma, found mainly in adolescents, routinely forms around the growth plate/metaphysis of long bones. Bone marrow composition changes with age, shifting from a more hematopoietic to an adipocyte-rich tissue. This conversion occurs in the metaphysis during adolescence, implicating a link between bone marrow conversion and osteosarcoma initiation. To
assess this, the tri-lineage differentiation potential of human bone marrow stromal cells (HBMSCs) isolated from the femoral diaphysis/metaphysis (FD) and epiphysis (FE) was characterized and compared to two osteosarcoma cell lines, Saos-2 and MG63. Compared to FE-cells, FD-cells showed an increase in tri-lineage differentiation. Additionally, differences were found between the Saos-2
cells exhibiting higher levels of osteogenic differentiation, lower adipogenic differentiation, and a more developed chondrogenic phenotype than MG63, with the Saos-2 being more comparable to FD-derived HBMSCs. The differences found between the FD and FE derived cells are consistent with the FD region
containing more hematopoietic tissue compared to the FE. This may be related to the similarities between FD-derived cells and Saos-2 cells during osteogenic and chondrogenic differentiation. These studies reveal distinct differences in the tri-lineage differentiations of ‘hematopoietic’ and ‘adipocyte rich’ bone
marrow, which correlate with specific characteristics of the two osteosarcoma cell lines.
Bone Marrow, Human Bone Marrow Stromal Cells, Osteosarcoma, Tri-lineage Differentiation
1422-0067
Smith, Hannah L.
e97b320e-8bee-4b80-84e5-d7da65e1e723
Gray, Juliet C.
12d5e17c-97bb-4d6d-8fc4-3914b730ed42
Beers, Stephen A.
a02548be-3ffd-41ab-9db8-d6e8c3b499a2
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Smith, Hannah L.
e97b320e-8bee-4b80-84e5-d7da65e1e723
Gray, Juliet C.
12d5e17c-97bb-4d6d-8fc4-3914b730ed42
Beers, Stephen A.
a02548be-3ffd-41ab-9db8-d6e8c3b499a2
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c

Smith, Hannah L., Gray, Juliet C., Beers, Stephen A. and Kanczler, Janos M. (2023) Tri-lineage differentiation potential of osteosarcoma cell lines and human bone marrow stromal cells from different anatomical locations. International Journal of Molecular Sciences, 24 (4), [3667]. (doi:10.3390/ijms24043667).

Record type: Article

Abstract

The bone cancer osteosarcoma, found mainly in adolescents, routinely forms around the growth plate/metaphysis of long bones. Bone marrow composition changes with age, shifting from a more hematopoietic to an adipocyte-rich tissue. This conversion occurs in the metaphysis during adolescence, implicating a link between bone marrow conversion and osteosarcoma initiation. To
assess this, the tri-lineage differentiation potential of human bone marrow stromal cells (HBMSCs) isolated from the femoral diaphysis/metaphysis (FD) and epiphysis (FE) was characterized and compared to two osteosarcoma cell lines, Saos-2 and MG63. Compared to FE-cells, FD-cells showed an increase in tri-lineage differentiation. Additionally, differences were found between the Saos-2
cells exhibiting higher levels of osteogenic differentiation, lower adipogenic differentiation, and a more developed chondrogenic phenotype than MG63, with the Saos-2 being more comparable to FD-derived HBMSCs. The differences found between the FD and FE derived cells are consistent with the FD region
containing more hematopoietic tissue compared to the FE. This may be related to the similarities between FD-derived cells and Saos-2 cells during osteogenic and chondrogenic differentiation. These studies reveal distinct differences in the tri-lineage differentiations of ‘hematopoietic’ and ‘adipocyte rich’ bone
marrow, which correlate with specific characteristics of the two osteosarcoma cell lines.

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Accepted/In Press date: 9 February 2023
Published date: 11 February 2023
Additional Information: Funding Information: Research funding for this study from Hannah’s Willberry Wonder Pony Charity (Registered Charity No: 1166416) and the University of Southampton (Presidential Scholarship Award 201819) is gratefully acknowledged. Publisher Copyright: © 2023 by the authors.
Keywords: Bone Marrow, Human Bone Marrow Stromal Cells, Osteosarcoma, Tri-lineage Differentiation

Identifiers

Local EPrints ID: 475594
URI: http://eprints.soton.ac.uk/id/eprint/475594
ISSN: 1422-0067
PURE UUID: 7462c9b9-4cc7-4f55-9a74-c7beb212bb7d
ORCID for Hannah L. Smith: ORCID iD orcid.org/0000-0001-7186-1981
ORCID for Juliet C. Gray: ORCID iD orcid.org/0000-0002-5652-4722
ORCID for Stephen A. Beers: ORCID iD orcid.org/0000-0002-3765-3342
ORCID for Janos M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414

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Date deposited: 22 Mar 2023 17:31
Last modified: 17 Mar 2024 03:47

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Contributors

Author: Hannah L. Smith ORCID iD
Author: Juliet C. Gray ORCID iD
Author: Janos M. Kanczler ORCID iD

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