Electrical broth micro-dilution for rapid antibiotic resistance testing
Electrical broth micro-dilution for rapid antibiotic resistance testing
Rapid tests to assess the susceptibility of bacteria to antibiotics are required to inform antibiotic stewardship. We have developed a novel test, which measures changes in the impedance of a 100 nanoliter volume of bacterial suspension to determine an “electrical” minimum inhibitory concentration (eMIC). Two representative strains of Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were tested against a panel of frontline antibiotics with different modes of action (ciprofloxacin, doxycycline, colistin and imipenem, gentamicin, and ceftazidime). The eMIC measured at 1 h correlated strongly with a standard 24 h microbroth dilution MIC for all combinations of antibiotics and bacteria, allowing strains to be correctly assigned as sensitive or resistant measured in a fraction of the time.
antimicrobial resistance, antimicrobial susceptibility test (AST), broth microdilution. minimum inhibitory concentration (MIC), impedance sensing
1101-1108
Spencer, Daniel Christopher
c84608fb-1c80-427c-aaa2-671bdb416085
Li, Yuetao
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Zhu, Yiling
a9e10808-eb8b-47f2-b1ac-906eca2e8555
Sutton, Mark
3ffaeca5-caf0-4d77-a573-fa4763387149
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
24 March 2023
Spencer, Daniel Christopher
c84608fb-1c80-427c-aaa2-671bdb416085
Li, Yuetao
32f69867-9a4d-4a10-b5a6-719cda207746
Zhu, Yiling
a9e10808-eb8b-47f2-b1ac-906eca2e8555
Sutton, Mark
3ffaeca5-caf0-4d77-a573-fa4763387149
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel Christopher, Li, Yuetao, Zhu, Yiling, Sutton, Mark and Morgan, Hywel
(2023)
Electrical broth micro-dilution for rapid antibiotic resistance testing.
ACS Sensors, 8 (3), .
(doi:10.1021/acssensors.2c02166).
Abstract
Rapid tests to assess the susceptibility of bacteria to antibiotics are required to inform antibiotic stewardship. We have developed a novel test, which measures changes in the impedance of a 100 nanoliter volume of bacterial suspension to determine an “electrical” minimum inhibitory concentration (eMIC). Two representative strains of Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were tested against a panel of frontline antibiotics with different modes of action (ciprofloxacin, doxycycline, colistin and imipenem, gentamicin, and ceftazidime). The eMIC measured at 1 h correlated strongly with a standard 24 h microbroth dilution MIC for all combinations of antibiotics and bacteria, allowing strains to be correctly assigned as sensitive or resistant measured in a fraction of the time.
Text
Electrical broth micro-dilution for rapid antibiotic resistance testing
- Accepted Manuscript
Text
acssensors.2c02166
- Version of Record
More information
Accepted/In Press date: 8 February 2023
e-pub ahead of print date: 23 February 2023
Published date: 24 March 2023
Additional Information:
Funding Information:
The authors would like to acknowledge funding from the Wessex Academic Health Science Network (AHSN).
Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.
Keywords:
antimicrobial resistance, antimicrobial susceptibility test (AST), broth microdilution. minimum inhibitory concentration (MIC), impedance sensing
Identifiers
Local EPrints ID: 476708
URI: http://eprints.soton.ac.uk/id/eprint/476708
ISSN: 2379-3694
PURE UUID: 38fa3d63-9a8b-4f4e-8599-812bb4e224b4
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Date deposited: 11 May 2023 17:01
Last modified: 17 Mar 2024 02:58
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Contributors
Author:
Daniel Christopher Spencer
Author:
Yuetao Li
Author:
Yiling Zhu
Author:
Mark Sutton
Author:
Hywel Morgan
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