Exogenous tetracosahexaenoic acid modifies the fatty acid composition of human primary T lymphocytes and Jurkat T cell leukemia cells contingent on cell type
Exogenous tetracosahexaenoic acid modifies the fatty acid composition of human primary T lymphocytes and Jurkat T cell leukemia cells contingent on cell type
Tetracosahexaenoic acid (24:6ω-3) is an intermediate in the conversion of 18:3ω-3 to 22:6ω-3 in mammals. There is limited information about whether cells can assimilate and metabolize exogenous 24:6ω-3. This study compared the effect of incubation with 24:6ω-3 on the fatty acid composition of two related cell types, primary CD3+ T lymphocytes and Jurkat T cell leukemia, which differ in the integrity of the polyunsaturated fatty acid (PUFA) biosynthesis pathway. 24:6ω-3 was only detected in either cell type when cells were incubated with 24:6ω-3. Incubation with 24:6ω-3 induced similar increments in the amount of 22:6ω-3 in both cell types and modified the homeoviscous adaptations fatty acid composition induced by activation of T lymphocytes. The effect of incubation with 18:3ω-3 compared to 24:6ω-3 on the increment in 22:6ω-3 was tested in Jurkat cells because primary T cells cannot convert 18:3ω-3 to 22:6ω-3. The increment in the 22:6ω-3 content of Jurkat cells incubated with 24:6ω-3 was 19.5-fold greater than that of cells incubated with 18:3ω-3. Acyl-coA oxidase siRNA knockdown decreased the amount of 22:6ω-3 and increased the amount of 24:6ω-3 in Jurkat cells. These findings show exogenous 24:6ω-3 can be incorporated into primary human T lymphocytes and Jurkat cells and induces changes in fatty acid composition consistent with its conversion to 22:6ω-3 via a mechanism involving peroxisomal β-oxidation that is regulated independently from the integrity of the upstream PUFA synthesis pathway. One further implication is that consuming 24:6ω-3 may be an effective alternative means of achieving health benefits attributed to 20:5ω-3 and 22:6ω-3.
desaturases, docosahexaenoic acid, fatty acid metabolism, general area, immunology, lipid biochemistry, mammalian lipid biochemistry, metabolism, n-3 fatty acids, polyunsaturated fatty acids (PUFA), specific lipids
185-196
Irvine, Nicola A.
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West, Annette L.
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Von Gerichten, Johanna
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Miles, Elizabeth A.
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Lillycrop, Karen A.
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Calder, Philip C.
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Fielding, Barbara A.
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Burdge, Graham
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July 2023
Irvine, Nicola A.
ed181be8-0435-49b7-bbc9-ddf2249fd2aa
West, Annette L.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Von Gerichten, Johanna
91e9d046-0eda-4425-9456-3edd372146a3
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Lillycrop, Karen A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Fielding, Barbara A.
abfc6274-5a80-43de-a5c0-ccef25005f39
Burdge, Graham
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Irvine, Nicola A., West, Annette L., Von Gerichten, Johanna, Miles, Elizabeth A., Lillycrop, Karen A., Calder, Philip C., Fielding, Barbara A. and Burdge, Graham
(2023)
Exogenous tetracosahexaenoic acid modifies the fatty acid composition of human primary T lymphocytes and Jurkat T cell leukemia cells contingent on cell type.
Lipids, 58 (4), .
(doi:10.1002/lipd.12372).
Abstract
Tetracosahexaenoic acid (24:6ω-3) is an intermediate in the conversion of 18:3ω-3 to 22:6ω-3 in mammals. There is limited information about whether cells can assimilate and metabolize exogenous 24:6ω-3. This study compared the effect of incubation with 24:6ω-3 on the fatty acid composition of two related cell types, primary CD3+ T lymphocytes and Jurkat T cell leukemia, which differ in the integrity of the polyunsaturated fatty acid (PUFA) biosynthesis pathway. 24:6ω-3 was only detected in either cell type when cells were incubated with 24:6ω-3. Incubation with 24:6ω-3 induced similar increments in the amount of 22:6ω-3 in both cell types and modified the homeoviscous adaptations fatty acid composition induced by activation of T lymphocytes. The effect of incubation with 18:3ω-3 compared to 24:6ω-3 on the increment in 22:6ω-3 was tested in Jurkat cells because primary T cells cannot convert 18:3ω-3 to 22:6ω-3. The increment in the 22:6ω-3 content of Jurkat cells incubated with 24:6ω-3 was 19.5-fold greater than that of cells incubated with 18:3ω-3. Acyl-coA oxidase siRNA knockdown decreased the amount of 22:6ω-3 and increased the amount of 24:6ω-3 in Jurkat cells. These findings show exogenous 24:6ω-3 can be incorporated into primary human T lymphocytes and Jurkat cells and induces changes in fatty acid composition consistent with its conversion to 22:6ω-3 via a mechanism involving peroxisomal β-oxidation that is regulated independently from the integrity of the upstream PUFA synthesis pathway. One further implication is that consuming 24:6ω-3 may be an effective alternative means of achieving health benefits attributed to 20:5ω-3 and 22:6ω-3.
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Irvine_Final_post_peer_review_27_April_2023
- Accepted Manuscript
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Lipids - 2023 - Irvine - Exogenous tetracosahexaenoic acid modifies the fatty acid composition of human primary T
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More information
Accepted/In Press date: 26 April 2023
e-pub ahead of print date: 13 May 2023
Published date: July 2023
Additional Information:
Funding Information:
Graham C. Burdge has received research funding from Nestle, Abbott Nutrition, and Danone and has served as a member of the Scientific Advisory Board of BASF. Philip C. Calder acts as a consultant to BASF, Smartfish, DSM, Cargill, Danone/Nutricia, and Fresenius‐Kabi. Karen A. Lillycrop has received research funding from Nestle, Abbott Nutrition, and Danone.
Funding Information:
This work was supported by grants from the Biotechnology and Biological Sciences Research Council (BB/S00548X/1 and BB/S005358/1). Philip C. Calder and Graham C. Burdge are supported by the National Institute for Health and Care Research through the NIHR Southampton Biomedical Research Centre. Neither funder was involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit the manuscript for publication. Publication costs were paid by the University of Southampton.
Funding Information:
This work was supported by grants from the Biotechnology and Biological Sciences Research Council (BB/S00548X/1 and BB/S005358/1). Philip C. Calder and Graham C. Burdge are supported by the National Institute for Health and Care Research through the NIHR Southampton Biomedical Research Centre. Neither funder was involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit the manuscript for publication. Publication costs were paid by the University of Southampton.
Publisher Copyright:
© 2023 The Authors. Lipids published by Wiley Periodicals LLC on behalf of AOCS.
Keywords:
desaturases, docosahexaenoic acid, fatty acid metabolism, general area, immunology, lipid biochemistry, mammalian lipid biochemistry, metabolism, n-3 fatty acids, polyunsaturated fatty acids (PUFA), specific lipids
Identifiers
Local EPrints ID: 477459
URI: http://eprints.soton.ac.uk/id/eprint/477459
ISSN: 0024-4201
PURE UUID: 15b9120f-2132-4586-9a7e-9b8f332d269d
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Date deposited: 06 Jun 2023 17:08
Last modified: 14 Aug 2024 01:34
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Contributors
Author:
Nicola A. Irvine
Author:
Annette L. West
Author:
Johanna Von Gerichten
Author:
Barbara A. Fielding
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