Protein encapsulation: A nanocarrier approach to the fluorescence imaging of an enzyme-based biomarker
Protein encapsulation: A nanocarrier approach to the fluorescence imaging of an enzyme-based biomarker
We report a new pentafluoropropanamido rhodamine fluorescent probe (ACS-HNE) that facilitates the selective detection of neutrophil elastase (NE). ACS-HNE displayed high sensitivity, with a low limit of detection (< 5.3 nM), and excellent selectivity towards elastase over other relevant biological analytes and enzymes. The comparatively poor solubility and cell permeability of ACS-HNE was improved by creating a ACS-HNE-albumin complex; this approach allowed for improvements in the in situ visualisation of elastase activity in RAW264.7 cells relative to ACS-HNE alone. As such the present study serves to demonstrate a simple strategy that may be used to overcome cell impermeability and solubility limitations, and to prepare probes suitable for the cellular imaging of enzymatic activity in vitro.
Jia, Zhiyuan
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Han, Hai-Hao Han
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Sedgwick, Adam C.
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Williams, George T.
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Gwynne, Lauren
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Brewster II, James T.
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Bull, Steven D.
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Jenkins, A. Toby A.
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He, Xiao-Peng
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Schönherr, Holger
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Sessler, Jonathan L.
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James, Tony D.
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3 June 2020
Jia, Zhiyuan
546a16a2-9213-40bb-8663-85d28a32f139
Han, Hai-Hao Han
adcd0f31-c774-4772-b098-567937b835af
Sedgwick, Adam C.
7329dac0-8976-4885-bbe9-6edb9d1ab017
Williams, George T.
26810522-92ef-4b61-a766-582bf15be280
Gwynne, Lauren
98d6197d-32de-42a3-bd48-7e21e1b877f2
Brewster II, James T.
cd239246-f30d-41e4-b5fd-937c950750a9
Bull, Steven D.
c5be72da-be12-4d0c-aae2-d3b1eb5a0c5d
Jenkins, A. Toby A.
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He, Xiao-Peng
d14ac412-8378-4611-aff7-5037f44ece14
Schönherr, Holger
bb46ea6d-8dcc-4fe8-8fbe-94804385702a
Sessler, Jonathan L.
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James, Tony D.
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Jia, Zhiyuan, Han, Hai-Hao Han, Sedgwick, Adam C., Williams, George T., Gwynne, Lauren, Brewster II, James T., Bull, Steven D., Jenkins, A. Toby A., He, Xiao-Peng, Schönherr, Holger, Sessler, Jonathan L. and James, Tony D.
(2020)
Protein encapsulation: A nanocarrier approach to the fluorescence imaging of an enzyme-based biomarker.
Frontiers in Chemistry, 8, [389].
(doi:10.3389/fchem.2020.00389).
Abstract
We report a new pentafluoropropanamido rhodamine fluorescent probe (ACS-HNE) that facilitates the selective detection of neutrophil elastase (NE). ACS-HNE displayed high sensitivity, with a low limit of detection (< 5.3 nM), and excellent selectivity towards elastase over other relevant biological analytes and enzymes. The comparatively poor solubility and cell permeability of ACS-HNE was improved by creating a ACS-HNE-albumin complex; this approach allowed for improvements in the in situ visualisation of elastase activity in RAW264.7 cells relative to ACS-HNE alone. As such the present study serves to demonstrate a simple strategy that may be used to overcome cell impermeability and solubility limitations, and to prepare probes suitable for the cellular imaging of enzymatic activity in vitro.
Text
fchem-08-00389
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Accepted/In Press date: 14 April 2020
Published date: 3 June 2020
Identifiers
Local EPrints ID: 478701
URI: http://eprints.soton.ac.uk/id/eprint/478701
ISSN: 2296-2646
PURE UUID: 36b721a0-a157-42aa-baed-1ad4bb211e49
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Date deposited: 07 Jul 2023 16:39
Last modified: 17 Mar 2024 04:17
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Contributors
Author:
Zhiyuan Jia
Author:
Hai-Hao Han Han
Author:
Adam C. Sedgwick
Author:
George T. Williams
Author:
Lauren Gwynne
Author:
James T. Brewster II
Author:
Steven D. Bull
Author:
A. Toby A. Jenkins
Author:
Xiao-Peng He
Author:
Holger Schönherr
Author:
Jonathan L. Sessler
Author:
Tony D. James
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