Novel ultra-fast pulsed laser sources for bio-imaging applications
Novel ultra-fast pulsed laser sources for bio-imaging applications
This thesis presents new techniques for the development of compact and reliable laser sources using advanced fibre laser technology, aiming to enhance the multiphoton microscopy (MPM) for biomedical imaging applications. Our work explores second-order multiphoton-based imaging systems, including second harmonic generation (SHG), two-photon exited fluorescence (TPEF), and coherent antiStokes Raman scattering (CARS) microscopy. These systems require picosecond (ps) pulsed lasers with specific spectral and tunability characteristics. I demonstrate a compact, continuous wave (CW) seeded, synchronization-free optical parametric amplifier (OPA), bypassing the complex cavity design of optical parametric oscillators (OPOs). The OPA with 175 mW power level, 8 cm−1 spectral resolution, and 2 ps pulse duration is optimized for CARS microscopy, with rapid and precise tuning achieved through the periodically-poled lithium niobate (PPLN) OPA crystal. We also delve into third-order multiphoton imaging and three-photon microscopy (3PM) systems, which offer improved resolution and penetration depth. These systems require ultrafast lase.
University of Southampton
Xu, Duanyang
2eeb11e3-d31e-4552-a925-49a3ffd7da20
14 September 2023
Xu, Duanyang
2eeb11e3-d31e-4552-a925-49a3ffd7da20
Richardson, David
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Xu, Lin
b887cecd-d21e-49f4-9b45-6909a7369e84
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Xu, Duanyang
(2023)
Novel ultra-fast pulsed laser sources for bio-imaging applications.
University of Southampton, Doctoral Thesis, 186pp.
Record type:
Thesis
(Doctoral)
Abstract
This thesis presents new techniques for the development of compact and reliable laser sources using advanced fibre laser technology, aiming to enhance the multiphoton microscopy (MPM) for biomedical imaging applications. Our work explores second-order multiphoton-based imaging systems, including second harmonic generation (SHG), two-photon exited fluorescence (TPEF), and coherent antiStokes Raman scattering (CARS) microscopy. These systems require picosecond (ps) pulsed lasers with specific spectral and tunability characteristics. I demonstrate a compact, continuous wave (CW) seeded, synchronization-free optical parametric amplifier (OPA), bypassing the complex cavity design of optical parametric oscillators (OPOs). The OPA with 175 mW power level, 8 cm−1 spectral resolution, and 2 ps pulse duration is optimized for CARS microscopy, with rapid and precise tuning achieved through the periodically-poled lithium niobate (PPLN) OPA crystal. We also delve into third-order multiphoton imaging and three-photon microscopy (3PM) systems, which offer improved resolution and penetration depth. These systems require ultrafast lase.
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Published date: 14 September 2023
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Local EPrints ID: 481950
URI: http://eprints.soton.ac.uk/id/eprint/481950
PURE UUID: 7037c4d5-d52f-450e-8922-3b4a186fbeed
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Date deposited: 14 Sep 2023 16:32
Last modified: 18 Mar 2024 03:26
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Contributors
Author:
Duanyang Xu
Thesis advisor:
Lin Xu
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