Ultrastructural cilia defects in multi-ciliated uterine glandular epithelial cells from women with reproductive failure
Ultrastructural cilia defects in multi-ciliated uterine glandular epithelial cells from women with reproductive failure
In brief: The causes of subfertility and recurrent pregnancy loss are often unclear. This study shows that endometrial gland cilia from women with subfertility have ultrastructural defects. Abstract: Endometrial glands secrete products into the endometrium and are necessary for embryo implantation and successful pregnancy. However, structural and functional abnormalities in endometrial gland cilia from women with reproductive failure remain poorly understood. This was a cross-sectional study where endometrial biopsies were collected at days 19-23 of the menstrual cycle from women with unexplained recurrent pregnancy loss (n = 15), unexplained subfertility (n = 11) or from egg donor control participants (n = 10). Endometrial gland cilia ultrastructure was imaged by transmission electron microscopy and cilia defects assessed by an electron-microscopist from a national primary ciliary dyskinesia diagnostic centre. Endometrial glands were isolated, and the cilia beat frequency recorded by high speed video. Subfertile women have proportionately lower ultrastructurally normal cilia (P < 0.05); higher frequency of absent dynamin arms (P < 0.01) or inner arm defects (P < 0.01) and lower cilia beat frequency (P < 0.05). The mechanisms underlying these obversions have yet to be determined. Recent studies have identified cilia related gene expression changes associated with reproductive failure and this study adds to the growing body of literature revealing structural and functional changes. The observation that cilia defects occurred at a higher frequency in endometrial glands of subfertile women raises the question of its mechanistic role in implantation.
Pearson-Farr, Jennifer E.
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Doherty, Regan
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Chatelet, David S.
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Goggin, Patricia
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Ng, Ka Ying Bonnie
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Lucas, Jane S.
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Cleal, Jane K.
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Cheong, Ying C.
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Lewis, Rohan M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
1 January 2024
Pearson-Farr, Jennifer E.
6c1e2bb4-ea4f-4fbe-a3fa-36a47757dc21
Doherty, Regan
da470b85-b358-4e8a-aec6-983ef87a89fc
Chatelet, David S.
6371fd7a-e274-4738-9ccb-3dd4dab32928
Goggin, Patricia
e1e6172b-6e76-47aa-a2a6-3f2a7f4a0a7d
Ng, Ka Ying Bonnie
869b3b2d-dd7c-407d-88aa-5db399e02b3a
Lucas, Jane S.
5cb3546c-87b2-4e59-af48-402076e25313
Cleal, Jane K.
18cfd2c1-bd86-4a13-b38f-c321af56da66
Cheong, Ying C.
4efbba2a-3036-4dce-82f1-8b4017952c83
Lewis, Rohan M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Pearson-Farr, Jennifer E., Doherty, Regan, Chatelet, David S., Goggin, Patricia, Ng, Ka Ying Bonnie, Lucas, Jane S., Cleal, Jane K., Cheong, Ying C. and Lewis, Rohan M.
(2024)
Ultrastructural cilia defects in multi-ciliated uterine glandular epithelial cells from women with reproductive failure.
Reproduction, 167 (1), [e230173].
(doi:10.1530/REP-23-0173).
Abstract
In brief: The causes of subfertility and recurrent pregnancy loss are often unclear. This study shows that endometrial gland cilia from women with subfertility have ultrastructural defects. Abstract: Endometrial glands secrete products into the endometrium and are necessary for embryo implantation and successful pregnancy. However, structural and functional abnormalities in endometrial gland cilia from women with reproductive failure remain poorly understood. This was a cross-sectional study where endometrial biopsies were collected at days 19-23 of the menstrual cycle from women with unexplained recurrent pregnancy loss (n = 15), unexplained subfertility (n = 11) or from egg donor control participants (n = 10). Endometrial gland cilia ultrastructure was imaged by transmission electron microscopy and cilia defects assessed by an electron-microscopist from a national primary ciliary dyskinesia diagnostic centre. Endometrial glands were isolated, and the cilia beat frequency recorded by high speed video. Subfertile women have proportionately lower ultrastructurally normal cilia (P < 0.05); higher frequency of absent dynamin arms (P < 0.01) or inner arm defects (P < 0.01) and lower cilia beat frequency (P < 0.05). The mechanisms underlying these obversions have yet to be determined. Recent studies have identified cilia related gene expression changes associated with reproductive failure and this study adds to the growing body of literature revealing structural and functional changes. The observation that cilia defects occurred at a higher frequency in endometrial glands of subfertile women raises the question of its mechanistic role in implantation.
Text
cilia paper resubmission authors accepted manuscript
- Accepted Manuscript
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e-pub ahead of print date: 1 November 2023
Published date: 1 January 2024
Identifiers
Local EPrints ID: 484961
URI: http://eprints.soton.ac.uk/id/eprint/484961
ISSN: 0022-4251
PURE UUID: 6865b8d3-458a-484b-adf0-4f3d171c5c0a
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Date deposited: 27 Nov 2023 17:32
Last modified: 16 Apr 2024 01:41
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Contributors
Author:
Jennifer E. Pearson-Farr
Author:
Regan Doherty
Author:
David S. Chatelet
Author:
Patricia Goggin
Author:
Ka Ying Bonnie Ng
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