Genome sequence and characterization of coliphage môr ffagbaw
Genome sequence and characterization of coliphage môr ffagbaw
Background: the quality of coastal waters around the United Kingdom is an area of increasing concern following sewer overflow, where wastewater is discharged into the environment. Coliphages, viruses that infect coliform bacteria, are associated with water quality in aquatic systems, yet remain largely uncharacterized at the genomic level.
Materials and methods: phage môr ffagbaw was isolated from seawater against Escherichia coli by enrichment and plaque assays. Whole genome sequencing, transmission electron microscopy, and host range analysis against the E. coli reference (ECOR) collection were used to characterize the phage.
Results: the virion had a siphovirus morphology and genomic analysis placed it within the family Drexlerviridae, subfamily Tempevirinae, and forms a new species within the genus Hanrivervirus. Spot assays revealed that phage môr ffagbaw could form plaques on 6 out of 72 ECOR strains (8%).
Conclusions: môr ffagbaw represents a new species of phage within the genus Hanrivervirus, with a narrow host range.
coliphage, genomes, phages
159-164
Thomas, Nerissa E.
679390f6-1a12-4734-96bc-01d576f94108
Ugokwe, Nzubechukwu Innocent
d6877ca4-cbf7-44ce-be54-1eab7bed568f
Kinsella, Andrew
71dd8d1d-fb8e-469b-bf6f-c44872a73875
Galyov, Edouard
86cd77f5-4b52-4834-b7f8-1797c2b7fb04
van Schaik, Willem
2a9ad6d2-6ba2-4345-84a9-8f85766667c2
Joyce, Naomi
c2ee0157-dc46-49fd-b04b-1461f6b30218
Nobrega, Franklin L.
6532795d-88a4-4f05-9b26-6af5b8f21a0d
Millard, Andrew
ce540fa4-62b7-4a06-9573-5eba937556a5
Wilkinson, Rachael C.
133e973f-3982-403b-b51e-0fecdf5ab23a
Thomas, Nerissa E.
679390f6-1a12-4734-96bc-01d576f94108
Ugokwe, Nzubechukwu Innocent
d6877ca4-cbf7-44ce-be54-1eab7bed568f
Kinsella, Andrew
71dd8d1d-fb8e-469b-bf6f-c44872a73875
Galyov, Edouard
86cd77f5-4b52-4834-b7f8-1797c2b7fb04
van Schaik, Willem
2a9ad6d2-6ba2-4345-84a9-8f85766667c2
Joyce, Naomi
c2ee0157-dc46-49fd-b04b-1461f6b30218
Nobrega, Franklin L.
6532795d-88a4-4f05-9b26-6af5b8f21a0d
Millard, Andrew
ce540fa4-62b7-4a06-9573-5eba937556a5
Wilkinson, Rachael C.
133e973f-3982-403b-b51e-0fecdf5ab23a
Thomas, Nerissa E., Ugokwe, Nzubechukwu Innocent, Kinsella, Andrew, Galyov, Edouard, van Schaik, Willem, Joyce, Naomi, Nobrega, Franklin L., Millard, Andrew and Wilkinson, Rachael C.
(2023)
Genome sequence and characterization of coliphage môr ffagbaw.
PHAGE, 4 (4), .
(doi:10.1089/phage.2023.0029).
Abstract
Background: the quality of coastal waters around the United Kingdom is an area of increasing concern following sewer overflow, where wastewater is discharged into the environment. Coliphages, viruses that infect coliform bacteria, are associated with water quality in aquatic systems, yet remain largely uncharacterized at the genomic level.
Materials and methods: phage môr ffagbaw was isolated from seawater against Escherichia coli by enrichment and plaque assays. Whole genome sequencing, transmission electron microscopy, and host range analysis against the E. coli reference (ECOR) collection were used to characterize the phage.
Results: the virion had a siphovirus morphology and genomic analysis placed it within the family Drexlerviridae, subfamily Tempevirinae, and forms a new species within the genus Hanrivervirus. Spot assays revealed that phage môr ffagbaw could form plaques on 6 out of 72 ECOR strains (8%).
Conclusions: môr ffagbaw represents a new species of phage within the genus Hanrivervirus, with a narrow host range.
Text
RevisedManuscript_Rachael
- Accepted Manuscript
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e-pub ahead of print date: 14 December 2023
Additional Information:
Funding Information:
Bioinformatics analysis was carried out using MRC CLIMB Infrastructure MR/L015080/1. R.C.W. and N.E.T. were funded by the European Structural Fund Programme 2014–2020 via the European Regional Development Fund funded C81844/C81845 ACCELERATE (Welsh Health Innovation Technology Accelerator) project. A.M. was funded by MR/L015080/1. N.I.U. and A.K. were in receipt of PhD studentships funded by the University of Leicester F100 and MRC-AIM DTP, respectively.
Publisher Copyright:
© Mary Ann Liebert, Inc.
Keywords:
coliphage, genomes, phages
Identifiers
Local EPrints ID: 486420
URI: http://eprints.soton.ac.uk/id/eprint/486420
ISSN: 2641-6530
PURE UUID: 277c4eba-f3f9-4978-b543-6f307757ef26
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Date deposited: 22 Jan 2024 17:32
Last modified: 18 Mar 2024 03:57
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Contributors
Author:
Nerissa E. Thomas
Author:
Nzubechukwu Innocent Ugokwe
Author:
Andrew Kinsella
Author:
Edouard Galyov
Author:
Willem van Schaik
Author:
Naomi Joyce
Author:
Andrew Millard
Author:
Rachael C. Wilkinson
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