Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.
Bacteriological Techniques/methods, Endotoxins/analysis, Gram-Negative Bacteria/chemistry, Lipid A/analysis, Sensitivity and Specificity, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation
68-71
Larrouy-Maumus, Gerald
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Clements, Abigail
540cdc04-276f-42bf-87a0-218bf4e75142
Filloux, Alain
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McCarthy, Ronan R
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Mostowy, Serge
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7 December 2015
Larrouy-Maumus, Gerald
6e4e5c7d-be07-49a8-af51-318157d37bc6
Clements, Abigail
540cdc04-276f-42bf-87a0-218bf4e75142
Filloux, Alain
6f79a42e-17d2-4fb1-bab9-a27c41a5b029
McCarthy, Ronan R
0b2cf2e0-b0ff-4c92-aa04-92d91182d1f2
Mostowy, Serge
879cc4d0-38b8-479b-a832-6d0d40d840ce
Larrouy-Maumus, Gerald, Clements, Abigail, Filloux, Alain, McCarthy, Ronan R and Mostowy, Serge
(2015)
Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
Journal of Microbiological Methods, 120, .
(doi:10.1016/j.mimet.2015.12.004).
Abstract
The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.
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More information
Accepted/In Press date: 2 December 2015
e-pub ahead of print date: 3 December 2015
Published date: 7 December 2015
Additional Information:
Copyright © 2015. Published by Elsevier B.V.
Keywords:
Bacteriological Techniques/methods, Endotoxins/analysis, Gram-Negative Bacteria/chemistry, Lipid A/analysis, Sensitivity and Specificity, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation
Identifiers
Local EPrints ID: 506046
URI: http://eprints.soton.ac.uk/id/eprint/506046
ISSN: 0167-7012
PURE UUID: 6013fb25-f89a-42e3-900d-7e8dfc358fb5
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Date deposited: 28 Oct 2025 17:34
Last modified: 29 Oct 2025 03:15
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Contributors
Author:
Gerald Larrouy-Maumus
Author:
Abigail Clements
Author:
Alain Filloux
Author:
Ronan R McCarthy
Author:
Serge Mostowy
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