The University of Southampton
University of Southampton Institutional Repository

Imaging of mice and men; adventures in multispectral imaging

Imaging of mice and men; adventures in multispectral imaging
Imaging of mice and men; adventures in multispectral imaging
Cancer of the brain and CNS account for only 2% of new cancer cases in the UK however it is responsible for 7% of cancer deaths of those aged under 70 years of age. Although surgery falls short of a cure it is the primary method of treatment. Two of the key problems in tumour surgery in the brain are a) that many tumours are visually indistinguishable from normal tissue even for experienced surgeons and b) that the risk of post-surgical neurological deficit is related to the proximity of functional (or 'eloquent') neurological tissue. In collaboration with surgeons at the Southampton University NHS Hospitals Trust we seek to address both of these problems. Firstly there is literature evidence that normal and neoplastic tissue have different spectral characteristics in the visible and near-infrared region. We investigate whether these can be practically imaged intraoperatively to establish disease state. Secondly the redox state of haemoglobin is known to affect it's visible and near-infrared spectral characteristics. This project investigates whether it is possible to identify the haemodynamic response associated with functional activity intraoperatively in the human brain. Prion diseases are fatal chronic neurodegenerative diseases of animals and man. They have gained notoriety due to recent outbreaks of Bovine Spongiform Encephalopathy (BSE) and the evidence that they can be transmitted between species, including to man. Exposure to BSE infected material has been shown to cause variant Creutzfeldt-Jacob disease in man. Prion disease is also used as a model of other neurodegenerative diseases, such as Alzheimers disease. Remarkably little is known about this class of disease including the specific cause of the neurodegeneration. Prions are a mis-folded protein which have a different conformation than the normal protein. Certain spectral features in the mid infrared region are associated with protein conformation. In collaboration with neuro-biologists within the university and using a synchrotron light source we investigate the application of multispectral imaging in early stage prion disease. By analysis of the protein conformation sensitivity of the mid infrared spectra (with particular interest in the Amide I band) we seek to identify structurally relevant markers in a mouse model before clinical symptoms of the disease are evident. This may lead to better understanding of the disease progression and the neurotoxic element.
Hoy, Paul R.
fefd720e-8c03-4acc-b9ac-a09c19165f1f
Hoy, Paul R.
fefd720e-8c03-4acc-b9ac-a09c19165f1f

Hoy, Paul R. (2009) Imaging of mice and men; adventures in multispectral imaging. University of Southampton, Optoelectronic Research Centre, Doctoral Thesis, 193pp.

Record type: Thesis (Doctoral)

Abstract

Cancer of the brain and CNS account for only 2% of new cancer cases in the UK however it is responsible for 7% of cancer deaths of those aged under 70 years of age. Although surgery falls short of a cure it is the primary method of treatment. Two of the key problems in tumour surgery in the brain are a) that many tumours are visually indistinguishable from normal tissue even for experienced surgeons and b) that the risk of post-surgical neurological deficit is related to the proximity of functional (or 'eloquent') neurological tissue. In collaboration with surgeons at the Southampton University NHS Hospitals Trust we seek to address both of these problems. Firstly there is literature evidence that normal and neoplastic tissue have different spectral characteristics in the visible and near-infrared region. We investigate whether these can be practically imaged intraoperatively to establish disease state. Secondly the redox state of haemoglobin is known to affect it's visible and near-infrared spectral characteristics. This project investigates whether it is possible to identify the haemodynamic response associated with functional activity intraoperatively in the human brain. Prion diseases are fatal chronic neurodegenerative diseases of animals and man. They have gained notoriety due to recent outbreaks of Bovine Spongiform Encephalopathy (BSE) and the evidence that they can be transmitted between species, including to man. Exposure to BSE infected material has been shown to cause variant Creutzfeldt-Jacob disease in man. Prion disease is also used as a model of other neurodegenerative diseases, such as Alzheimers disease. Remarkably little is known about this class of disease including the specific cause of the neurodegeneration. Prions are a mis-folded protein which have a different conformation than the normal protein. Certain spectral features in the mid infrared region are associated with protein conformation. In collaboration with neuro-biologists within the university and using a synchrotron light source we investigate the application of multispectral imaging in early stage prion disease. By analysis of the protein conformation sensitivity of the mid infrared spectra (with particular interest in the Amide I band) we seek to identify structurally relevant markers in a mouse model before clinical symptoms of the disease are evident. This may lead to better understanding of the disease progression and the neurotoxic element.

Text
Hoy_2009_thesis_4446.pdf - Other
Download (9MB)

More information

Published date: 2009
Organisations: University of Southampton

Identifiers

Local EPrints ID: 70911
URI: http://eprints.soton.ac.uk/id/eprint/70911
PURE UUID: d7b73085-d171-4598-9a58-8e2808129b5b

Catalogue record

Date deposited: 11 Dec 2009
Last modified: 29 Jan 2020 13:04

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×