The University of Southampton
University of Southampton Institutional Repository

Linking root structure to functionality: the impact of root system architecture on citrate enhanced phosphate uptake

Linking root structure to functionality: the impact of root system architecture on citrate enhanced phosphate uptake
Linking root structure to functionality: the impact of root system architecture on citrate enhanced phosphate uptake

Root citrate exudation is thought to be important for phosphate solubilization. Previous research has concluded that cluster-like roots benefit most from this exudation in terms of increased phosphate uptake, suggesting that root structure plays an important role in citrate-enhanced uptake (additional phosphate uptake due to citrate exudation). Time-resolved computed tomography images of wheat root systems were used as the geometry for 3D citrate-phosphate solubilization models. Citrate-enhanced uptake was correlated with morphological measures of the root systems to determine which had the most benefit. A large variation of citrate-enhanced uptake over 11 root structures was observed. Root surface area dominated absolute phosphate uptake, but did not explain citrate-enhanced uptake. Number of exuding root tips correlated well with citrate-enhanced uptake. Root tips in close proximity could collectively exude high amounts of citrate, resulting in a delayed spike in citrate-enhanced uptake. Root system architecture plays an important role in citrate-enhanced uptake. Singular morphological measurements of the root systems cannot entirely explain variations in citrate-enhanced uptake. Root systems with many tips would benefit greatly from citrate exudation. Quantifying citrate-enhanced uptake experimentally is difficult as variations in root surface area would overwhelm citrate benefits.

Root system architecture, a-biotic stress, citrate, exudation, modelling, phosphorus
0028-646X
376-391
Mckay Fletcher, Daniel
db06e7e0-69af-4fa2-89b3-26f6599e43d4
Ruiz, Siul Aljadi
d79b3b82-7c0d-47cc-9616-11d29e6a41bd
Gerheim Souza Dias, Tiago
cbb905da-3e22-4933-8b7b-663c52345e3a
Petroselli, Chiara
19266726-2dc0-4790-af77-7ccdc45865eb
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Mckay Fletcher, Daniel
db06e7e0-69af-4fa2-89b3-26f6599e43d4
Ruiz, Siul Aljadi
d79b3b82-7c0d-47cc-9616-11d29e6a41bd
Gerheim Souza Dias, Tiago
cbb905da-3e22-4933-8b7b-663c52345e3a
Petroselli, Chiara
19266726-2dc0-4790-af77-7ccdc45865eb
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Mckay Fletcher, Daniel, Ruiz, Siul Aljadi, Gerheim Souza Dias, Tiago, Petroselli, Chiara and Roose, Tiina (2020) Linking root structure to functionality: the impact of root system architecture on citrate enhanced phosphate uptake. New Phytologist, 227 (2), 376-391. (doi:10.1111/nph.16554).

Record type: Article

Abstract

Root citrate exudation is thought to be important for phosphate solubilization. Previous research has concluded that cluster-like roots benefit most from this exudation in terms of increased phosphate uptake, suggesting that root structure plays an important role in citrate-enhanced uptake (additional phosphate uptake due to citrate exudation). Time-resolved computed tomography images of wheat root systems were used as the geometry for 3D citrate-phosphate solubilization models. Citrate-enhanced uptake was correlated with morphological measures of the root systems to determine which had the most benefit. A large variation of citrate-enhanced uptake over 11 root structures was observed. Root surface area dominated absolute phosphate uptake, but did not explain citrate-enhanced uptake. Number of exuding root tips correlated well with citrate-enhanced uptake. Root tips in close proximity could collectively exude high amounts of citrate, resulting in a delayed spike in citrate-enhanced uptake. Root system architecture plays an important role in citrate-enhanced uptake. Singular morphological measurements of the root systems cannot entirely explain variations in citrate-enhanced uptake. Root systems with many tips would benefit greatly from citrate exudation. Quantifying citrate-enhanced uptake experimentally is difficult as variations in root surface area would overwhelm citrate benefits.

Text
manuscript_Ammended_proofedits - Accepted Manuscript
Download (2MB)
Text
SupplementaryMaterial_proofedits - Accepted Manuscript
Download (319kB)

More information

Accepted/In Press date: 3 March 2020
e-pub ahead of print date: 21 March 2020
Published date: 1 July 2020
Additional Information: Funding Information: DMMF, CP and TR are funded by ERC Consolidator grant 646809 (Data Intensive Modelling of the Rhizosphere Processes). SR and TR are funded by BBSRC SARISA BB/L025620/1, BBSRC SARIC BB/P004180/1 and NERC NE/L00237/1; TR also is funded by EPSRC EP/M020355/1. We would like to thank Chris Topp (Danforth Center, USA) for discussions on plant phenotyping and function which contributed to this manuscript. Publisher Copyright: © 2020 The Authors. New Phytologist © 2020 New Phytologist Trust
Keywords: Root system architecture, a-biotic stress, citrate, exudation, modelling, phosphorus

Identifiers

Local EPrints ID: 438879
URI: http://eprints.soton.ac.uk/id/eprint/438879
ISSN: 0028-646X
PURE UUID: ce481dbd-2f92-49b2-8eeb-1bcfc23dda8a
ORCID for Daniel Mckay Fletcher: ORCID iD orcid.org/0000-0001-6569-2931
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 26 Mar 2020 17:30
Last modified: 17 Mar 2024 05:25

Export record

Altmetrics

Contributors

Author: Daniel Mckay Fletcher ORCID iD
Author: Tiago Gerheim Souza Dias
Author: Chiara Petroselli
Author: Tiina Roose ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×