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Epigenome-wide association study reveals duration of breastfeeding is associated with epigenetic differences in children

Epigenome-wide association study reveals duration of breastfeeding is associated with epigenetic differences in children
Epigenome-wide association study reveals duration of breastfeeding is associated with epigenetic differences in children
Several small studies have shown associations between breastfeeding and genome-wide DNA methylation (DNAm). We performed a comprehensive Epigenome-Wide Association Study (EWAS) to identify associations between breastfeeding and DNAm patterns in childhood. We analysed DNAm data from the Isle of Wight Birth Cohort at birth, 10, 18 and 26 years. The feeding method was categorized as breastfeeding duration >3 months and >6 months, and exclusive breastfeeding duration >3 months. EWASs using robust linear regression were performed to identify differentially methylated positions (DMPs) in breastfed and non-breastfed children at age 10 (false discovery rate of 5%). Differentially methylated regions (DMRs) were identified using comb-p. The persistence of significant associations was evaluated in neonates and individuals at 18 and 26 years. Two DMPs, in genes SNX25 and LINC00840, were significantly associated with breastfeeding duration >6 months at 10 years and was replicated for >3 months of exclusive breastfeeding. Additionally, a significant DMR spanning the gene FDFT1 was identified in 10-year-old children who were exposed to a breastfeeding duration >3 months. None of these signals persisted to 18 or 26 years. This study lends further support for a suggestive role of DNAm in the known benefits of breastfeeding on a child’s future health.
Breastfeeding, Breastfeeding duration, DNA methylation, EWAS, Epigenetics, Epigenome-wide association study
1660-4601
Sherwood, William B.
49cd2f0d-ef0c-4b2a-88e3-02915058f28f
Kothalawala, Dilini, Mahesha
c22b9e92-e60a-44b6-a34b-2eb37a3a1212
Perunthadambil Kadalayil, Latha
e620b801-844a-45d9-acaf-e0a58acd7cf2
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Karmaus, Wilfried
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Arshad, Syed
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Rezwan, Faisal I
203f8f38-1f5d-485b-ab11-c546b4276338
Sherwood, William B.
49cd2f0d-ef0c-4b2a-88e3-02915058f28f
Kothalawala, Dilini, Mahesha
c22b9e92-e60a-44b6-a34b-2eb37a3a1212
Perunthadambil Kadalayil, Latha
e620b801-844a-45d9-acaf-e0a58acd7cf2
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853
Arshad, Syed
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Rezwan, Faisal I
203f8f38-1f5d-485b-ab11-c546b4276338

Sherwood, William B., Kothalawala, Dilini, Mahesha, Perunthadambil Kadalayil, Latha, Ewart, Susan, Zhang, Hongmei, Karmaus, Wilfried, Arshad, Syed, Holloway, John and Rezwan, Faisal I (2020) Epigenome-wide association study reveals duration of breastfeeding is associated with epigenetic differences in children. International Journal of Environmental Research and Public Health, 17 (10), [3569]. (doi:10.3390/ijerph17103569).

Record type: Article

Abstract

Several small studies have shown associations between breastfeeding and genome-wide DNA methylation (DNAm). We performed a comprehensive Epigenome-Wide Association Study (EWAS) to identify associations between breastfeeding and DNAm patterns in childhood. We analysed DNAm data from the Isle of Wight Birth Cohort at birth, 10, 18 and 26 years. The feeding method was categorized as breastfeeding duration >3 months and >6 months, and exclusive breastfeeding duration >3 months. EWASs using robust linear regression were performed to identify differentially methylated positions (DMPs) in breastfed and non-breastfed children at age 10 (false discovery rate of 5%). Differentially methylated regions (DMRs) were identified using comb-p. The persistence of significant associations was evaluated in neonates and individuals at 18 and 26 years. Two DMPs, in genes SNX25 and LINC00840, were significantly associated with breastfeeding duration >6 months at 10 years and was replicated for >3 months of exclusive breastfeeding. Additionally, a significant DMR spanning the gene FDFT1 was identified in 10-year-old children who were exposed to a breastfeeding duration >3 months. None of these signals persisted to 18 or 26 years. This study lends further support for a suggestive role of DNAm in the known benefits of breastfeeding on a child’s future health.

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More information

Accepted/In Press date: 17 May 2020
Published date: 20 May 2020
Additional Information: William B Sherwood is a student at the University of Southampton but PURE doesn't recognise his name, his email address is: ws2g14@soton.ac.uk
Keywords: Breastfeeding, Breastfeeding duration, DNA methylation, EWAS, Epigenetics, Epigenome-wide association study

Identifiers

Local EPrints ID: 440951
URI: http://eprints.soton.ac.uk/id/eprint/440951
ISSN: 1660-4601
PURE UUID: c791493c-c496-4911-85be-8c5ace5b9e8b
ORCID for John Holloway: ORCID iD orcid.org/0000-0001-9998-0464
ORCID for Faisal I Rezwan: ORCID iD orcid.org/0000-0001-9921-222X

Catalogue record

Date deposited: 26 May 2020 16:30
Last modified: 26 Nov 2021 03:00

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Contributors

Author: William B. Sherwood
Author: Dilini, Mahesha Kothalawala
Author: Susan Ewart
Author: Hongmei Zhang
Author: Wilfried Karmaus
Author: Syed Arshad
Author: John Holloway ORCID iD
Author: Faisal I Rezwan ORCID iD

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