Selective depletion of radiolabeled HER2-specific antibody for contrast improvement during PET
Selective depletion of radiolabeled HER2-specific antibody for contrast improvement during PET
The prolonged in vivo persistence of antibodies results in high background and poor contrast during their use as molecular imaging agents for positron emission tomography (PET). We have recently described a class of engineered Fc fusion proteins that selectively deplete antigen-specific antibodies without affecting the levels of antibodies of other specificities. Here, we demonstrate that these Fc fusions (called Seldegs, for selective degradation) can be used to clear circulating, radiolabeled HER2-specific antibody during diagnostic imaging of HER2-positive tumors in mice. The analyses show that Seldegs have considerable promise for the reduction of whole-body exposure to radiolabel and improvement of contrast during PET.
Engineered Fc fusions, HER2, PET
Khare, Priyanka
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Sun, Wei
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Ramakrishnan, Sreevidhya
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Swiercz, Rafal
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Hao, Guiyang
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Lo, Su-Tang
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Nham, Kien
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Sun, Xiankai
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Ober, R.J.
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Ward, E. Sally
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30 September 2021
Khare, Priyanka
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Sun, Wei
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Ramakrishnan, Sreevidhya
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Swiercz, Rafal
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Hao, Guiyang
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Lo, Su-Tang
4113a11a-d1a6-49f2-bad3-49d1ab64376d
Nham, Kien
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Sun, Xiankai
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Ober, R.J.
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Ward, E. Sally
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Khare, Priyanka, Sun, Wei, Ramakrishnan, Sreevidhya, Swiercz, Rafal, Hao, Guiyang, Lo, Su-Tang, Nham, Kien, Sun, Xiankai, Ober, R.J. and Ward, E. Sally
(2021)
Selective depletion of radiolabeled HER2-specific antibody for contrast improvement during PET.
mAbs, 13 (1), [1976705].
(doi:10.1080/19420862.2021.1976705).
Abstract
The prolonged in vivo persistence of antibodies results in high background and poor contrast during their use as molecular imaging agents for positron emission tomography (PET). We have recently described a class of engineered Fc fusion proteins that selectively deplete antigen-specific antibodies without affecting the levels of antibodies of other specificities. Here, we demonstrate that these Fc fusions (called Seldegs, for selective degradation) can be used to clear circulating, radiolabeled HER2-specific antibody during diagnostic imaging of HER2-positive tumors in mice. The analyses show that Seldegs have considerable promise for the reduction of whole-body exposure to radiolabel and improvement of contrast during PET.
Text
19420862.2021
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supplementary data_081621
More information
Accepted/In Press date: 1 September 2021
e-pub ahead of print date: 30 September 2021
Published date: 30 September 2021
Keywords:
Engineered Fc fusions, HER2, PET
Identifiers
Local EPrints ID: 451648
URI: http://eprints.soton.ac.uk/id/eprint/451648
ISSN: 1942-0862
PURE UUID: 2d4c92bf-fabc-4f60-9bd5-a7b373857a9a
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Date deposited: 18 Oct 2021 16:31
Last modified: 17 Mar 2024 03:53
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Contributors
Author:
Priyanka Khare
Author:
Wei Sun
Author:
Sreevidhya Ramakrishnan
Author:
Rafal Swiercz
Author:
Guiyang Hao
Author:
Su-Tang Lo
Author:
Kien Nham
Author:
Xiankai Sun
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