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Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'

Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'
Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'
This dataset contains two databases demonstrating the raw data collected for measuring plasmid stabilty in C. muridarum strains. The first dataset contains data for quantifying the frequency of plasmid loss as a proxy for the rate of plasmid loss, by quantifying the proportion of plasmid-free inclusions in the total population identified by the absence of GFP expressed in trans. The second dataset contains qPCR data measured from samples of total genomic DNA extracted from harvested inocula at passage 3 and 6 only, for determination of plasmid copy number (absolute plasmid copies per chromosome). The qPCR protocol used primer pairs to target single copy genes located on the chromosome (omcB) and on the plasmid (CDS2).
plasmid copy number, plasmid loss frequency, Chlamydia muridarum
University of Southampton
Cousins, Emma
6c252521-4654-4189-8564-59cdf631b380
Cousins, Emma
6c252521-4654-4189-8564-59cdf631b380

Cousins, Emma (2022) Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'. University of Southampton doi:10.5258/SOTON/D2425 [Dataset]

Record type: Dataset

Abstract

This dataset contains two databases demonstrating the raw data collected for measuring plasmid stabilty in C. muridarum strains. The first dataset contains data for quantifying the frequency of plasmid loss as a proxy for the rate of plasmid loss, by quantifying the proportion of plasmid-free inclusions in the total population identified by the absence of GFP expressed in trans. The second dataset contains qPCR data measured from samples of total genomic DNA extracted from harvested inocula at passage 3 and 6 only, for determination of plasmid copy number (absolute plasmid copies per chromosome). The qPCR protocol used primer pairs to target single copy genes located on the chromosome (omcB) and on the plasmid (CDS2).

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thesis_readme_Dataset_in_support_of_Southampton_doctoral_thesis.txt - Dataset
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Plasmid_loss_frequency_in_Chlamydia_muridarum_strains.xlsx - Dataset
Available under License Creative Commons Attribution.
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Plasmid_copy_number_ratios_all_strains.xlsx - Dataset
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More information

Published date: 2 November 2022
Keywords: plasmid copy number, plasmid loss frequency, Chlamydia muridarum

Identifiers

Local EPrints ID: 471407
URI: http://eprints.soton.ac.uk/id/eprint/471407
PURE UUID: 39891c81-1518-4436-bc04-5a1c65592c2c

Catalogue record

Date deposited: 07 Nov 2022 18:03
Last modified: 07 Nov 2022 18:07

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Contributors

Creator: Emma Cousins

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