Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'
Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'
This dataset contains two databases demonstrating the raw data collected for measuring plasmid stabilty in C. muridarum strains.
The first dataset contains data for quantifying the frequency of plasmid loss as a proxy for the rate of plasmid loss, by quantifying the proportion of plasmid-free inclusions in the total population identified by the absence of GFP expressed in trans.
The second dataset contains qPCR data measured from samples of total genomic DNA extracted from harvested inocula at passage 3 and 6 only, for determination of plasmid copy number (absolute plasmid copies per chromosome). The qPCR protocol used primer pairs to target single copy genes located on the chromosome (omcB) and on the plasmid (CDS2).
plasmid copy number, plasmid loss frequency, Chlamydia muridarum
University of Southampton
Cousins, Emma
6c252521-4654-4189-8564-59cdf631b380
Cousins, Emma
6c252521-4654-4189-8564-59cdf631b380
Cousins, Emma
(2022)
Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'.
University of Southampton
doi:10.5258/SOTON/D2425
[Dataset]
Abstract
This dataset contains two databases demonstrating the raw data collected for measuring plasmid stabilty in C. muridarum strains.
The first dataset contains data for quantifying the frequency of plasmid loss as a proxy for the rate of plasmid loss, by quantifying the proportion of plasmid-free inclusions in the total population identified by the absence of GFP expressed in trans.
The second dataset contains qPCR data measured from samples of total genomic DNA extracted from harvested inocula at passage 3 and 6 only, for determination of plasmid copy number (absolute plasmid copies per chromosome). The qPCR protocol used primer pairs to target single copy genes located on the chromosome (omcB) and on the plasmid (CDS2).
Text
thesis_readme_Dataset_in_support_of_Southampton_doctoral_thesis.txt
- Dataset
Spreadsheet
Plasmid_loss_frequency_in_Chlamydia_muridarum_strains.xlsx
- Dataset
Spreadsheet
Plasmid_copy_number_ratios_all_strains.xlsx
- Dataset
More information
Published date: 2 November 2022
Keywords:
plasmid copy number, plasmid loss frequency, Chlamydia muridarum
Identifiers
Local EPrints ID: 471407
URI: http://eprints.soton.ac.uk/id/eprint/471407
PURE UUID: 39891c81-1518-4436-bc04-5a1c65592c2c
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Date deposited: 07 Nov 2022 18:03
Last modified: 05 May 2023 20:08
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Contributors
Creator:
Emma Cousins
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