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Systemic lupus erythematosus: disease activity may influence the release of endothelial microparticles?

Systemic lupus erythematosus: disease activity may influence the release of endothelial microparticles?
Systemic lupus erythematosus: disease activity may influence the release of endothelial microparticles?
To evaluate blood-borne endothelial microparticles (EMPs) in women with SLE and correlated these to disease activity as defined by the SLEDAI-2K score. The study takes cross-sectional design. A total of 90 age-matched women were recruited including: G1 (healthy volunteers, n = 30), G2 (women with SLE and low disease activity (SLEDAI-2K score ≤4; n = 30) and G3 (women with SLE and moderate/high disease activity (SLEDAI-2K score >4; n = 30). Blood was collected in 3.2% sodium citrate. Subsequently, the microparticles were purified by ultracentrifugation and labeled with anti-CD51/61 and anti-Annexin-V antibodies. Quantification and phenotyping were performed using flow cytometry. The number of EMPs was significantly higher in SLE patients compared with controls (P = 0.0178). When SLE patients were stratified according to disease activity, the number of EMPs was significantly increased in women with moderate-to-high disease activity compared with controls (P = 0.0074). We observed a correlation between the number of EMPs and age (r = −0.34; P = 0.0123) and between the number of EMPs and SLEDAI-2K score (r = 0.30; P = 0.04). Our results suggest that the SLE causes increased EMPs release, especially in patients with SLEDAI-2K score greater than 4. Although measurement of the EMPs could be useful in distinguishing patients with SLE from health controls, they have limited value in differentiating between SLE subtypes.
1473-5733
189-195
Cicarini, Walter B.
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Ferreira, Karine S.
0c7df9c4-e1cc-40ad-b307-0a86fa16f464
Loures, Cristina M.G.
d521c03e-a4b3-4932-ac12-7e2b5d2d3cfb
Consoli, Renato V.
f8b421db-8e65-4e36-931f-ae6126f3778a
Neiva, Claudia L.S.
cea5ae75-e263-42af-89db-ee2118c78fc2
Padua, Paulo M.
cf3ba259-08d5-4e89-9283-639db6b5440b
Nunes, Fernanda F.C.
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Alves, Luan C.V.
e3219f41-f831-4a18-a20e-62498a88f95a
Reis, Edna A.
082e12d7-10a4-48fc-b6a4-f36cac97857b
Toledo, Vincente P.C.P.
601e1045-2f45-4541-a8c3-427923bca78b
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Carvalho, Maria G.
b7495b77-148e-47f5-88f2-f613b58be29f
Cicarini, Walter B.
781091cf-19ab-456c-9084-cba37dc0b3f9
Ferreira, Karine S.
0c7df9c4-e1cc-40ad-b307-0a86fa16f464
Loures, Cristina M.G.
d521c03e-a4b3-4932-ac12-7e2b5d2d3cfb
Consoli, Renato V.
f8b421db-8e65-4e36-931f-ae6126f3778a
Neiva, Claudia L.S.
cea5ae75-e263-42af-89db-ee2118c78fc2
Padua, Paulo M.
cf3ba259-08d5-4e89-9283-639db6b5440b
Nunes, Fernanda F.C.
0fba803b-27de-485c-83ef-43f3d5af9e22
Alves, Luan C.V.
e3219f41-f831-4a18-a20e-62498a88f95a
Reis, Edna A.
082e12d7-10a4-48fc-b6a4-f36cac97857b
Toledo, Vincente P.C.P.
601e1045-2f45-4541-a8c3-427923bca78b
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Carvalho, Maria G.
b7495b77-148e-47f5-88f2-f613b58be29f

Cicarini, Walter B., Ferreira, Karine S., Loures, Cristina M.G., Consoli, Renato V., Neiva, Claudia L.S., Padua, Paulo M., Nunes, Fernanda F.C., Alves, Luan C.V., Reis, Edna A., Toledo, Vincente P.C.P., Lwaleed, Bashir A. and Carvalho, Maria G. (2018) Systemic lupus erythematosus: disease activity may influence the release of endothelial microparticles? Blood Coagulation & Fibrinolysis, 29 (2), 189-195. (doi:10.1097/MBC.0000000000000703).

Record type: Article

Abstract

To evaluate blood-borne endothelial microparticles (EMPs) in women with SLE and correlated these to disease activity as defined by the SLEDAI-2K score. The study takes cross-sectional design. A total of 90 age-matched women were recruited including: G1 (healthy volunteers, n = 30), G2 (women with SLE and low disease activity (SLEDAI-2K score ≤4; n = 30) and G3 (women with SLE and moderate/high disease activity (SLEDAI-2K score >4; n = 30). Blood was collected in 3.2% sodium citrate. Subsequently, the microparticles were purified by ultracentrifugation and labeled with anti-CD51/61 and anti-Annexin-V antibodies. Quantification and phenotyping were performed using flow cytometry. The number of EMPs was significantly higher in SLE patients compared with controls (P = 0.0178). When SLE patients were stratified according to disease activity, the number of EMPs was significantly increased in women with moderate-to-high disease activity compared with controls (P = 0.0074). We observed a correlation between the number of EMPs and age (r = −0.34; P = 0.0123) and between the number of EMPs and SLEDAI-2K score (r = 0.30; P = 0.04). Our results suggest that the SLE causes increased EMPs release, especially in patients with SLEDAI-2K score greater than 4. Although measurement of the EMPs could be useful in distinguishing patients with SLE from health controls, they have limited value in differentiating between SLE subtypes.

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Accepted/In Press date: 19 December 2017
e-pub ahead of print date: 12 January 2018
Published date: March 2018

Identifiers

Local EPrints ID: 417930
URI: https://eprints.soton.ac.uk/id/eprint/417930
ISSN: 1473-5733
PURE UUID: b1ba1fa9-d42a-4207-aa1f-919f8a6f9372

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Date deposited: 16 Feb 2018 17:31
Last modified: 14 Mar 2019 05:18

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