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Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease

Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Plasma periostin was analysed using ELISA in 144 paediatric patients and 38 controls. Plasma levels were assessed against validated disease activity indices in IBD and clinical outcomes. An immuno-fluorescence for periostin and detailed isoform-expression analysis in the colonic tissue was performed in 23 individuals. We integrated a whole-gene based burden metric ‘GenePy’ to assess the impact of variation in POSTN and 23 other genes functionally connected to periostin. We found that plasma periostin levels were significantly increased during remission compared to active Crohn’s disease. The immuno-fluorescence analysis demonstrated enhanced peri-cryptal ring patterns in patients compared to controls, present throughout inflamed, as well as macroscopically non-inflamed colonic tissue. Interestingly, the pattern of isoforms remained unchanged during bowel inflammation compared to healthy controls. In addition to its role during the inflammatory processes in IBD, periostin may have an additional prominent role in mucosal repair. Additional studies will be necessary to understand its role in the pathogenesis, repair and fibrosis in IBD.
2045-2322
6194
Coelho, Tracy
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Riethmacher, Eva
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Gao, Yifang
eea234ba-f566-4f21-a65e-234b84cba285
Mossotto, Enrico
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Khojanazarov, Alisher
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Griffin, Ann
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Mukanova, Saida
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Ashimkanova, Aiymkul
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Haggarty, Rachel
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Borissenko, Anton
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Ashton, James
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Stafford, Imogen Sian
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Batra, Akshay
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Afzal, Nadeem A.
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Stanton, Michael P.
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Vadgama, Bhumita
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Adrisova, Kapura
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Beattie, Robert
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Williams, Anthony
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Ennis, Sarah
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Riethmacher, Dieter
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Coelho, Tracy
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Riethmacher, Eva
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Gao, Yifang
eea234ba-f566-4f21-a65e-234b84cba285
Mossotto, Enrico
a2a572db-3e95-41c6-94f6-f1b019594372
Khojanazarov, Alisher
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Griffin, Ann
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Mukanova, Saida
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Ashimkanova, Aiymkul
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Haggarty, Rachel
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Borissenko, Anton
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Ashton, James
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Stafford, Imogen Sian
50987dc1-3772-408f-9093-9124f3d6b2cd
Batra, Akshay
822f891e-87ca-41d9-b68d-27c395e88809
Afzal, Nadeem A.
62505946-2503-42ba-9b02-85513bb3ec87
Stanton, Michael P.
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Vadgama, Bhumita
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Adrisova, Kapura
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Beattie, Robert
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Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Ennis, Sarah
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Riethmacher, Dieter
1a0a0c2e-e94d-4d0a-a890-90107a2545bc

Coelho, Tracy, Riethmacher, Eva, Gao, Yifang, Mossotto, Enrico, Khojanazarov, Alisher, Griffin, Ann, Mukanova, Saida, Ashimkanova, Aiymkul, Haggarty, Rachel, Borissenko, Anton, Ashton, James, Stafford, Imogen Sian, Batra, Akshay, Afzal, Nadeem A., Stanton, Michael P., Vadgama, Bhumita, Adrisova, Kapura, Beattie, Robert, Williams, Anthony, Ennis, Sarah and Riethmacher, Dieter (2021) Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease. Scientific Reports, 11 (1), 6194, [6194]. (doi:10.1038/s41598-021-85096-7).

Record type: Article

Abstract

The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Plasma periostin was analysed using ELISA in 144 paediatric patients and 38 controls. Plasma levels were assessed against validated disease activity indices in IBD and clinical outcomes. An immuno-fluorescence for periostin and detailed isoform-expression analysis in the colonic tissue was performed in 23 individuals. We integrated a whole-gene based burden metric ‘GenePy’ to assess the impact of variation in POSTN and 23 other genes functionally connected to periostin. We found that plasma periostin levels were significantly increased during remission compared to active Crohn’s disease. The immuno-fluorescence analysis demonstrated enhanced peri-cryptal ring patterns in patients compared to controls, present throughout inflamed, as well as macroscopically non-inflamed colonic tissue. Interestingly, the pattern of isoforms remained unchanged during bowel inflammation compared to healthy controls. In addition to its role during the inflammatory processes in IBD, periostin may have an additional prominent role in mucosal repair. Additional studies will be necessary to understand its role in the pathogenesis, repair and fibrosis in IBD.

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Accepted/In Press date: 25 February 2021
e-pub ahead of print date: 18 March 2021
Published date: 18 March 2021

Identifiers

Local EPrints ID: 447679
URI: http://eprints.soton.ac.uk/id/eprint/447679
ISSN: 2045-2322
PURE UUID: 9035906c-8c70-4bb5-9556-50363a2c4158
ORCID for Sarah Ennis: ORCID iD orcid.org/0000-0003-2648-0869
ORCID for Dieter Riethmacher: ORCID iD orcid.org/0000-0002-4206-5529

Catalogue record

Date deposited: 18 Mar 2021 17:43
Last modified: 26 Nov 2021 02:52

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Contributors

Author: Tracy Coelho
Author: Eva Riethmacher
Author: Yifang Gao
Author: Enrico Mossotto
Author: Alisher Khojanazarov
Author: Ann Griffin
Author: Saida Mukanova
Author: Aiymkul Ashimkanova
Author: Rachel Haggarty
Author: Anton Borissenko
Author: James Ashton
Author: Imogen Sian Stafford
Author: Akshay Batra
Author: Nadeem A. Afzal
Author: Michael P. Stanton
Author: Bhumita Vadgama
Author: Kapura Adrisova
Author: Robert Beattie
Author: Sarah Ennis ORCID iD
Author: Dieter Riethmacher ORCID iD

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